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Bead based immunoassay for mouse cytokines and chemokines

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The Bead-based immunoassay for mouse cytokines and chemokines is a lab equipment product that can be used to measure the levels of various proteins in mouse samples. It utilizes beads coated with specific antibodies to capture and detect the target analytes.

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Lab products found in correlation

Cd11b positive enrichment beads, by Miltenyi Biotec (2 mentions) Mopc 21, by BioXCell (2 mentions)

2 protocols using bead based immunoassay for mouse cytokines and chemokines

1

Aged Microglia Response to Amyloid-beta

Cited 1 time
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Microglia from aged (20–24 m.o.) mice were isolated as described above, with minor modifications. Namely, following myelin depletion, cells were incubated with CD11b-positive enrichment beads (Miltenyi), isolated by magnetic selection using LS-columns, and plated at 30,000 cells / well of a 96-well plate. Microglia were maintained in serum-free defined medium as previously described21 (link), and treated with either IgG (MOPC21, BioXCell, 20 μg/mL) or anti-CD22 (Cy34, BioXCell, 20 μg/mL) in the presence or absence of Aβ oligomers (5 μM) for 8 hours. Following incubation, supernatant was collected, spun down to remove cells, and flash frozen on dry ice. A bead-based immunoassay for mouse cytokines and chemokines (Eve Technologies) was used for protein detection.
Pluvinage J.V., Haney M.S., Smith B.A., Sun J., Iram T., Bonanno L., Li L., Lee D.P., Morgens D.W., Yang A.C., Shuken S.R., Gate D., Scott M., Khatri P., Luo J., Bertozzi C.R., Bassik M.C, & Wyss-Coray T. (2019). CD22 blockade restores homeostatic microglial phagocytosis in aging brains. Nature, 568(7751), 187-192.
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2

Aged Microglia Response to Amyloid-beta

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Microglia from aged (20–24 m.o.) mice were isolated as described above, with minor modifications. Namely, following myelin depletion, cells were incubated with CD11b-positive enrichment beads (Miltenyi), isolated by magnetic selection using LS-columns, and plated at 30,000 cells / well of a 96-well plate. Microglia were maintained in serum-free defined medium as previously described21 (link), and treated with either IgG (MOPC21, BioXCell, 20 μg/mL) or anti-CD22 (Cy34, BioXCell, 20 μg/mL) in the presence or absence of Aβ oligomers (5 μM) for 8 hours. Following incubation, supernatant was collected, spun down to remove cells, and flash frozen on dry ice. A bead-based immunoassay for mouse cytokines and chemokines (Eve Technologies) was used for protein detection.
Pluvinage J.V., Haney M.S., Smith B.A., Sun J., Iram T., Bonanno L., Li L., Lee D.P., Morgens D.W., Yang A.C., Shuken S.R., Gate D., Scott M., Khatri P., Luo J., Bertozzi C.R., Bassik M.C, & Wyss-Coray T. (2019). CD22 blockade restores homeostatic microglial phagocytosis in aging brains. Nature, 568(7751), 187-192.
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