Mca1774f

Manufactured by Bio-Rad

The MCA1774F is a laboratory instrument manufactured by Bio-Rad. It is designed to perform a core function related to the analysis and processing of biological samples. No further details are available without the risk of making unsubstantiated claims.

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Lab products found in correlation

Percp efluor 710, by Thermo Fisher Scientific (1 mentions) Fixable viability dye 780, by Thermo Fisher Scientific (1 mentions) Goat anti mouse igg r pe, by Jackson ImmunoResearch (1 mentions)

2 protocols using mca1774f

Multiparametric Immune Cell Analysis

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CD3-FITC (clone CA17.2A12, BioRad #MCA1774F), NKp46-PE (clone 48A, kind gift of Dr. Dean Lee), CD5 on PerCP-eFluor 710 (clone YKIX322.3) Thermo Fisher #46-5050-42, Live/dead staining performed using Fixable Viability Dye 780 (eBioscience #65-0865-14).

Gingrich A.A., Reiter T.E., Judge S.J., York D., Yanagisawa M., Razmara A., Sturgill I., Basmaci U.N., Brady R.V., Stoffel K., Murphy W.J., Rebhun R.B., Brown C.T, & Canter R.J. (2021). Comparative Immunogenomics of Canine Natural Killer Cells as Immunotherapy Target. Frontiers in Immunology, 12, 670309.

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Characterization of Canine Immune Cells

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Blocking was performed in blocking buffer (50% FBS/PBS) with 0.1 mg of dog gamma globulin (Jackson ImmunoResearch, 0004-000-002) per 1,000,000 cells. Cells were stained with 200 ng/1,000,000 cells of mouse anti-canine NKp46 Clone 48A (isotype: IgG2a) or unconjugated TCRαβ/TCRγδ for 30 min, followed by goat anti-mouse IgG RPE (Jackson ImmunoResearch, 115-116-146) in 50% FBS/PBS, and finally with anti-canine TCRαβ/γδ (FITC/Alexa Fluor 488 when indicated; Peter Moore, UC Davis) and commercially available antibodies (Biorad) to canine CD3 (MCA1774F; clone CA17.2A12), CD8α (MCA1039A700; clone YCATE55.9), CD4 (MCA1038PECY7; clone YKIX302.9), CD21 (MCA1781A647; clone CA2.1D6), CD5 (MCA1037APC; YKIX322.3), and anti-human CD14 (MCA1568A647, MCA1568A700; TÜK4). Cells events were acquired on a LSR Fortessa. Flow cytometry gating was determined using cells stained with secondary only and single color controls were analyzed using FlowJo 7.6.5/10.

Foltz J.A., Somanchi S.S., Yang Y., Aquino-Lopez A., Bishop E.E, & Lee D.A. (2016). NCR1 Expression Identifies Canine Natural Killer Cell Subsets with Phenotypic Similarity to Human Natural Killer Cells. Frontiers in Immunology, 7, 521.

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