Brain tissue was homogenized using Agilent ceramic beads with a Beadblaster 24 homogenizer (Benchmark Scientific, Sayreville, NJ) and placed at −20 °C until extraction. Brain homogenate, serum, and standards were processed in acetonitrile at 4 °C, and then the supernatant was transferred, evaporated, and diluted in phosphate buffer. Samples were extracted using Bond Elut Plexa PCX extraction cartridges (Agilent, Santa Clara, CA), evaporated, and reconstituted in a solution of water, ammonium formate, and formic acid. Samples were injected onto a reversed-phase Agilent (Santa Clara, CA) Zorbax Eclipse plus C18 column (2.1 mm × 50 mm i.d., 1.8 μm) and then analyzed on an Agilent G6470A triple quadrupole LCMS/MS system consisting of an Infinity II 1290 G7116B Multicolumn Thermostat, G7120A High Speed Quad Pumps, and a G7267B Multisampler. Data acquisition and peak integration were analyzed using Mass Hunter software (Tokyo, Japan). Detailed methods are provided in the Supporting Materials .
Infinity 2 1290 g7116b multicolumn thermostat
Manufactured by Agilent Technologies
Sourced in Canada
The Infinity II 1290 G7116B Multicolumn Thermostat is a laboratory equipment designed to precisely control the temperature of multiple chromatography columns. It maintains a stable temperature environment to ensure consistent and reliable chromatographic separations.
Automatically generated - may contain errors
3 protocols using infinity 2 1290 g7116b multicolumn thermostat
1
Brain Metabolite Extraction and Analysis
2
Quantification of Oxycodone and Fentanyl
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Concentrations of oxycodone and fentanyl in brain and serum samples were determined by either single quadrupole GC/MS as described previously14 (link), or by an LCMS/MS system consisting of an Agilent G6470A TQ with an Infinity II 1290 G7116B Multicolumn Thermostat, G7120A High Speed Quad Pumps, G7267B Multisampler. Data acquisition and peak integration were analyzed using Mass Hunter software (Tokyo, Japan).
3
Quantitative Analysis of Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Samples were diluted in an acetonitrile solution (1:3) and centrifuged at 8609 x g for 10 min. The supernatant was transferred, evaporated, and diluted 1:1 in PBS. Samples were extracted using Bond Elut Plexa PCX, 3mL extraction cartridges (Agilent), evaporated, and reconstituted in a solution of H2O:0.1% ammonium formate:0.01% formic acid. Sample were injected onto a reversed phase Agilent Zorbax Eclipse plus C18 column (2.1 mm × 50 mm i.d., 1.8 μm). The LCMS/MS system consisted of an Agilent G6470A TQ with an Infinity II 1290 G7116B Multicolumn Thermostat, G7120A High Speed Quad Pumps, G7267B Multisampler. Data were analyzed using Mass Hunter software.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!