When cell membranes are compromised or damaged, the enzyme lactate dehydrogenase (L-LDH) is released into the surrounding extracellular space. The presence of this enzyme in the culture medium can be used as a cell death marker. To assess the release of L-LDH from biological samples, JEG-3 cell culture supernatants were incubated with the following buffer solution: NAD+ (Roche, Meylan, France; 0.75 mM), Lactic acid sodium salt (Sigma Aldrich, Henri Desbruères, France; 1 mM), Hydrazine monohydrate (Sigma; 0.4 M) and Glycine (EUROMEDEX; 0.4 M). LDH activity in samples was determined kinetically by monitoring NADH absorbance at 340 nm for 90 min using a microplate reader.
Lactic acid sodium salt
Manufactured by Merck Group
Sourced in United States
Lactic acid sodium salt is a chemical compound that serves as a source of lactic acid in various applications. It is a white, crystalline solid that is soluble in water. The core function of lactic acid sodium salt is to provide a stable and readily available form of lactic acid for use in scientific, industrial, and pharmaceutical settings.
Automatically generated - may contain errors
Lab products found in correlation
Potassium phosphate, by Merck Group (2 mentions)
Potassium citrate, by Merck Group (2 mentions)
Porcine gastric mucin type 2, by Merck Group (2 mentions)
Uric acid sodium salt, by Merck Group (2 mentions)
Sodium chloride (nacl), by Merck Group (2 mentions)
Urea, by Merck Group (2 mentions)
Glycine, by Euromedex (1 mentions)
Hydrazine monohydrate, by Merck Group (1 mentions)
Sodium phosphate monobasic, by Merck Group (1 mentions)
Sodium phosphate dibasic, by Merck Group (1 mentions)
Ammonium acetate, by Merck Group (1 mentions)
Acetic acid, by Merck Group (1 mentions)
Ammonium nitrate, by Merck Group (1 mentions)
Calcofluor white, by Merck Group (1 mentions)
Formaldehyde, by Merck Group (1 mentions)
Phosphate buffer saline (pbs), by Merck Group (1 mentions)
Potassium chloride (kcl), by Merck Group (1 mentions)
Calcium chloride, by Avantor (1 mentions)
3 protocols using lactic acid sodium salt
1
Quantifying Extracellular LDH Release
2
Quantifying Tomato Ripening Biomarkers
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Acetic acid, ammonium acetate, ammonium nitrate, dibasic sodium phosphate, Calcofluor white, formaldehyde, lactic acid sodium salt, monobasic sodium phosphate, porcine gastric mucin Type II, phosphate buffer saline, potassium citrate, potassium chloride, potassium phosphate, sodium chloride, urea, uric acid sodium salt, alcohol oxidase from Pichia pastoris (14 units/mg) were purchased from Sigma Chemical Co., St. Louis, MO, USA.. Acetylacetone at analytical grade was purchased from SLS, Nottingham, UK.
Salad tomatoes (Lycopersicum esculentum) at red light ripening stage were purchased from local supermarket (Tesco, UK).
Salad tomatoes (Lycopersicum esculentum) at red light ripening stage were purchased from local supermarket (Tesco, UK).
3
Artificial Saliva Gel Preparation
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Food grade quality kappa-carrageenan, locust bean gum and sodium alginate were purchased from Special Ingredients Ltd (Chesterfield, UK). Green food colouring was obtained from AmeriColor (Placentia, USA) and American peppermint extract was purchased at a local supermarket (Leeds, UK). Potassium chloride was purchased from Minerals Water Ltd (Purfleet, UK) and calcium chloride from VWR International (Leuven, Belgium). Additionally, sodium chloride, potassium phosphate, potassium citrate, uric acid sodium salt, urea, lactic acid sodium salt, and porcine gastric Mucin Type II were obtained from Sigma-Aldrich (St. Louis, USA). All materials were used without further purification. Demineralised water was used in preparation for all the gels and the artificial saliva formulation.
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