Anti phos akt

Manufactured by Cell Signaling Technology

Anti-phos-AKT is a laboratory reagent used to detect and quantify the phosphorylation state of the AKT protein. It is a specific antibody that recognizes the phosphorylated form of AKT, which is a key signaling molecule involved in various cellular processes.

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Anti-AKT (1447 citations) Phos-Akt (8 citations) Pho-Akt (2 citations)

2 protocols using anti phos akt

Sertoli Cell Protein Expression Analysis

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The cultured human Sertoli cells at different passages were lysed with RIPA buffer (Santa Cruz) for 30 min on ice. After 30 min lysis on ice, cell lysates were cleared by centrifugation at 12,000 g, and the concentration of protein was measured by BCA kit (Dingguo Company, China). Thirty micrograms of cell lysate from each sample were used for SDS-PAGE (Bio-Rad Laboratories, Richmond, CA), and Western blots were performed according to the protocol as described previously [11 (link)]. The chosen antibodies included anti-SCF (Sigma), anti-GDNF (Santa Cruz), anti-BMP4 (Abcam), anti-PCNA (Santa Cruz), anti-phos-AKT (Cell Signaling), and anti-phos-SMAD1/5 (Cell Signaling). After extensive washes with PBS, the blots were detected by chemiluminescence (Chemi-Doc XRS, Bio-Rad), and the integrated density values (IDV) was calculated by comparing the signals of target proteins with that of housekeeper ACTB (Santa Cruz).

Guo Y., Hai Y., Yao C., Chen Z., Hou J., Li Z, & He Z. (2015). Long-term culture and significant expansion of human Sertoli cells whilst maintaining stable global phenotype and AKT and SMAD1/5 activation. Cell Communication and Signaling : CCS, 13, 20.

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Pancreatic Marker Antibody Validation

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Pancreatic marker Kit (Catalogue #8679; anti-α-amylase, anti-keratin, anti-PLA-2GB), anti-pERK, anti-phos-AKT, anti-BRAF, anti-Bcl2, anti-Ki67 and anti-β-actin were purchased from Cell Signaling technology (Danvers, MA) whereas anti-KRAS-GTP antibody was procured from New East Biosciences (Malvern, PA).

Ganaie A.A., Siddique H.R., Sheikh I.A., Parray A., Wang L., Panyam J., Villalta P.W., Deng Y., Konety B.R, & Saleem M. (2020). A novel terpenoid class for prevention and treatment of KRAS-driven cancers: Comprehensive analysis using in situ, in vitro and in vivo model systems. Molecular carcinogenesis, 59(8), 886-896.

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