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Anhydrous ethanol solution

Manufactured by Merck Group

Anhydrous ethanol solution is a laboratory-grade solvent used for various scientific applications. It is a clear, colorless liquid consisting of 100% pure ethanol, with no added water content. This product is designed to meet the stringent purity requirements of research and analytical settings.

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2 protocols using anhydrous ethanol solution

1

Electrochemical Biosensor Fabrication

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30% hydrogen peroxide (H2O2), potassium nitrate (KNO3), ethanolamine, amine-PEG2-biotin, 1-ethyl-3-(3-(dimethylamino)propyl)-carbodiimide HCl (EDC), and potassium ferricyanide (Fe-(cn)63–) were purchased from Thermofisher Scientific (Fairlawn, NJ, USA). Anhydrous ethanol solution, 2-(N-morpholino)ethanesulfonic acid (MES) buffer, sulfo-N-hydroxsulfosuccinimide (NHS), lipoic acid, 11-mercaptoundecanoic acid (MUA), and potassium hexacyanoferrate(II) trihydrate (Fe(CN)64–) were purchased from Sigma-Aldrich (St. Louis, MO). 6-(Ferrocenyl)hexanethiol (Fc-(CH)6SH) was purchased from Santa Cruz Biotech (Dallas, TX). Sodium hydroxide (NaOH) was purchased from JT Baker (TX, USA). All solutions were prepared in ultrapure Milli-Q water (18.2 MΩ cm, MilliPore, MA, USA). Whatman 1 chromatography paper was purchased from GE healthcare sciences (UK). Scotch Heavy Duty Shipping packing tape (3M) was purchased from Office Max. Streptavidin (SA) microparticles were purchased from Life Technologies. High purity silver paint was purchased from SPI Supplies (West Chester, PA). Au microwires (99.99% pure, 25 μm diameter) were purchased from California Fine Wire Company (Grover Beach, CA). 0.05 M pH 6.0 MES buffer was made with MES, and pH was adjusted to 6.0 with NaOH.
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2

Synthesis of Ferrous Compounds

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Ferrous sulfate (99% AR, Sigma-Aldrich), sodium ferro-cyanide (99% AR, Sigma-Aldrich), aniline, AA (99.5% RT, Sigma-Aldrich) and anhydrous ethanol solution (ACS, ≥99.5%, Sigma-Aldrich) were used. Deionized water was used in all the synthesis process.
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