Apc conjugated anti human hla a2 antibody

Manufactured by BioLegend

The APC-conjugated anti-human HLA-A2 antibody is a fluorescently labeled monoclonal antibody that specifically binds to the HLA-A2 allele expressed on the surface of human cells. This product is designed for use in flow cytometry applications.

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Lab products found in correlation

Isoton 2 diluent, by Beckman Coulter (1 mentions) Gallios flow cytometer, by Beckman Coulter (1 mentions) Ma900, by Sony (1 mentions)

Close spelling variants of this product

Anti-human HLA-A2 Anti-HLA-A2 antibody

2 protocols using apc conjugated anti human hla a2 antibody

Phenotypic Characterization of HLA-A2 Cells

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Surface staining was performed to characterise target cells phenotypically to detect HLA-A2 expression using an (APC-conjugated anti-human HLA-A2 antibody (BioLegend, 343308, Clone: BB7.2). Phenotypic analysis was also performed to assess the success of HHDII gene transfection using an FITC-conjugated anti-human β2m antibody (Sigma-Aldrich, SAB4700012, Clone: β2M-01). Cells were incubated with PI immediately prior to running the samples. At the end of the assay, cells were re-suspended in Beckman Coulter Isoton II™ diluent and analysed immediately on the Beckman Coulter Gallios™ flow cytometer.

Almshayakhchi R., Nagarajan D., Vadakekolathu J., Guinn B.A., Reeder S., Brentville V., Metheringham R., Pockley A.G., Durrant L, & McArdle S. (2021). A Novel HAGE/WT1-ImmunoBody® Vaccine Combination Enhances Anti-Tumour Responses When Compared to Either Vaccine Alone. Frontiers in Oncology, 11, 636977.

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Sorting MHC Class I Surface Positive Cells

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For EpiScan screens, 30 μl of antibody (APC-conjugated anti-human HLA-A2 antibody, BioLegend, 343308 or APC-conjugated anti-human B2-microglobulin antibody, BioLegend, 316312) in a total volume of 1.5 ml was used per 10 million cells. Staining was conducted for 30 min at 4°C; cells were then washed in PBS prior to sorting. Sorting was performed on a Sony MA900 instrument. For EpiScan screen sorts, the small remaining library negative population was used to draw the MHC class I surface positive gate. Within that gate, four bins subdivided the positive population into equal populations. Examples can be found in Extended Data Fig. 4.

Ballestrem C., Wehrle-Haller B., Hinz B, & Imhof B.A. (2000). Actin-dependent Lamellipodia Formation and Microtubule-dependent Tail Retraction Control-directed Cell Migration. Molecular Biology of the Cell, 11(9), 2999-3012.

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