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Sc 55579

Manufactured by Santa Cruz Biotechnology
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Sourced in United States

Sc-55579 is a laboratory equipment product offered by Santa Cruz Biotechnology. It is designed for use in scientific research and analysis. The core function of this product is to facilitate specific laboratory procedures, but a detailed description cannot be provided while maintaining an unbiased and factual approach. Further information may be available upon request.

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Lab products found in correlation

A11008, by Thermo Fisher Scientific (2 mentions) Anti cortactin mouse, by Santa Cruz Biotechnology (2 mentions) A1978, by Merck Group (2 mentions) T5168, by Merck Group (2 mentions) Sc398326, by Santa Cruz Biotechnology (2 mentions) Universal immune peroxidase anti mouse, by Nichirei Biosciences (2 mentions) T5326, by Merck Group (2 mentions) Phalloidin ifluor 647 reagent, by Abcam (2 mentions) T5192, by Merck Group (2 mentions) Phalloidin ifluor 488 reagent, by Abcam (2 mentions)

3 protocols using sc 55579

1

Antibody Staining Protocol for Cellular Analysis

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Anti-ORP3 rabbit antibody (1:2000 for WB and 1:400 for IF, A304-557A, Bethyl Laboratories, Texas, USA). Anti-ORP3 mouse antibody (1:400 for IHC and 1:2000 for WB, sc398326, Santa Cruz, California, USA). Anti-Cortactin mouse antibody (1:500 for IF, sc-55579, Santa Cruz, California, USA). Anti-α-tubulin mouse antibody (1:2000 for WB, T5168, Sigma-Aldrich, St. Louis, USA). Anti-γ-tubulin mouse antibody (1:2000 for WB and 1:500 for IF, T5326, Sigma-Aldrich, St. Louis, USA). Anti-γ-tubulin rabbit antibody (1:500 for IF, T5192, Sigma-Aldrich, St. Louis, USA). Anti-β-actin mouse antibody (1:10,000 for WB, A1978, Sigma-Aldrich, St. Louis, USA). Secondary antibodies, HRP anti-Mouse antibody (1:5000 for WB, 7076S, Cell Signaling Technology, Danvers, USA) and HRP anti-Rabbit (1:2000 for WB, 7074S, Cell Signaling Technology, Danvers, USA). One drop of Universal immune-peroxidase anti-Mouse (Nichirei Bioscience, Tokyo, JP) per piece of tissue was used in IHC. Phalloidin-iFluor 488 Reagent (1:1000 for IF, ab176753, Abcam, Cambridge, UK). Phalloidin-iFluor647 reagent (1: 1000 for IF, ab176759, Abcam, Cambridge, UK). Anti-Rabbit conjugated with Alexa Fluor488 antibody (1:1000 for IF, A11008, ThermoFisher Scientific, Waltham, USA), and anti-Mouse conjugated with Cy5 antibody (1:200 for IF, 115–605-006, ThermoFisher Scientific, Waltham, USA).
Wang X., Liu J., Azoitei A., Eiseler T., Meessen S., Jiang W., Zheng X., Makori A.W., Eckstein M., Hartmann A., Stilgenbauer S., Elati M., Hohwieler M., Kleger A., John A., Zengerling F., Wezel F., Bolenz C, & Günes C. (2023). Loss of ORP3 induces aneuploidy and promotes bladder cancer cell invasion through deregulated microtubule and actin dynamics. Cellular and Molecular Life Sciences, 80(10), 299.
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2

Invadopodia Measurement in 3D Melanoma

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The number and length of the invadopodia of the 3D cultured WM35 cells were measured after 5 days or 10 days of culture with BRAF inhibition with 100 nM PLX4032 or with 0.1% DMSO. Bright field images of the 3D cultured spheroids were taken at z = 300 μm. From the brightfield images, the number and length of the invadopodia-like structure were analyzed using Fiji software. To acquire immunofluorescence images of the invadopodia of the cells cultured in 2D hydrogels or in 3D hydrogels, the WM35 cells that were 2D cultured after a day of PLX4032 treatment and the 3D encapsulated cells cultured in a normal culture medium for 1 day and then treated with 100nM PLX4032 for 4 days were fixed in 4% paraformaldehyde for 30 min at room temperature. The fixed samples were washed twice with PBS, permeabilized with PBST (0.1% TWEEN-20 in PBS) for 1 hour, incubated with 1% BSA in PBST for another 1 hour, and then incubated with a primary antibody of cortactin (sc-55579, Santa Cruz Biotechnology). Finally, the F-actin was stained with rhodamine-phalloidin, and the nuclear DNA was stained with 4’−6-diamidino-2-phenylindole (DAPI).
Shin D.S., Schroeder M.E, & Anseth K.S. (2021). Impact of collagen triple helix structure on melanoma cell invadopodia formation and matrix degradation upon BRAF inhibitor treatment. Advanced healthcare materials, 11(7), e2101592.
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3

Antibody Staining Protocol for Cellular Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Anti-ORP3 rabbit antibody (1:2000 for WB and 1:400 for IF, A304-557A, Bethyl Laboratories, Texas, USA). Anti-ORP3 mouse antibody (1:400 for IHC and 1:2000 for WB, sc398326, Santa Cruz, California, USA). Anti-Cortactin mouse antibody (1:500 for IF, sc-55579, Santa Cruz, California, USA). Anti-α-tubulin mouse antibody (1:2000 for WB, T5168, Sigma-Aldrich, St. Louis, USA). Anti-γ-tubulin mouse antibody (1:2000 for WB and 1:500 for IF, T5326, Sigma-Aldrich, St. Louis, USA). Anti-γ-tubulin rabbit antibody (1:500 for IF, T5192, Sigma-Aldrich, St. Louis, USA). Anti-β-actin mouse antibody (1:10,000 for WB, A1978, Sigma-Aldrich, St. Louis, USA). Secondary antibodies, HRP anti-Mouse antibody (1:5000 for WB, 7076S, Cell Signaling Technology, Danvers, USA) and HRP anti-Rabbit (1:2000 for WB, 7074S, Cell Signaling Technology, Danvers, USA). One drop of Universal immune-peroxidase anti-Mouse (Nichirei Bioscience, Tokyo, JP) per piece of tissue was used in IHC. Phalloidin-iFluor 488 Reagent (1:1000 for IF, ab176753, Abcam, Cambridge, UK). Phalloidin-iFluor647 reagent (1: 1000 for IF, ab176759, Abcam, Cambridge, UK). Anti-Rabbit conjugated with Alexa Fluor488 antibody (1:1000 for IF, A11008, ThermoFisher Scientific, Waltham, USA), and anti-Mouse conjugated with Cy5 antibody (1:200 for IF, 115–605-006, ThermoFisher Scientific, Waltham, USA).
Wang X., Liu J., Azoitei A., Eiseler T., Meessen S., Jiang W., Zheng X., Makori A.W., Eckstein M., Hartmann A., Stilgenbauer S., Elati M., Hohwieler M., Kleger A., John A., Zengerling F., Wezel F., Bolenz C, & Günes C. (2023). Loss of ORP3 induces aneuploidy and promotes bladder cancer cell invasion through deregulated microtubule and actin dynamics. Cellular and Molecular Life Sciences, 80(10), 299.
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