Rabbit anti axl

Manufactured by Cell Signaling Technology

Rabbit anti-AXL is a primary antibody product that recognizes the AXL receptor tyrosine kinase. AXL is a membrane-bound receptor that plays a role in cell signaling processes. This antibody can be used for the detection and analysis of AXL in various research applications.

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Lab products found in correlation

Lsm 710, by Zeiss (2 mentions) Duolink in situ detection reagents farred, by Merck Group (1 mentions) Axio imager z1 microscope, by Zeiss (1 mentions) Rat anti f4 80 sc 59171, by Santa Cruz Biotechnology (1 mentions) Abc hrp kit, by Vector Laboratories (1 mentions) 3 3 diaminobenzidine (dab), by Merck Group (1 mentions) Aquatex, by Merck Group (1 mentions) Donkey anti mouse 488, by Jackson ImmunoResearch (1 mentions) Prolong gold mounting medium, by Thermo Fisher Scientific (1 mentions) Mouse anti mitf, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Anti-AXL (11 citations) Anti-AXL C89E7 rabbit mAb (2 citations) Rabbit anti-AXL (C89E7, (2 citations)

3 protocols using rabbit anti axl

Proximity Ligation Assay for AXL-pY Interaction

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Proximity Ligation Assays (PLA) were performed as described (35 ). Briefly, cells were plated, fixed and permeabilized in eight-well chamber slides. Non-specific binding was blocked, followed by overnight incubation at 4°C with primary antibodies, rabbit anti-AXL (Cell Signaling 8661) and mouse anti-pY (pY-100; Cell Signaling 9411). Subsequent incubations with rabbit (+) and mouse (–) PLA probes and detection with Duolink In Situ Detection Reagents FarRed (Sigma) were carried out as per the manufacturer’s protocol. Fluorescent images were acquired on a fully automated upright Zeiss Axio Imager Z.1 microscope at 40X magnification using Axiovision software suite (version 4.6, Carl Zeiss Inc.).

Majumder A., Hosseinian S., Stroud M., Adhikari E., Saller J.J., Smith M.A., Zhang G., Agarwal S., Creixell M., Meyer B.S., Kinose F., Bowers K., Fang B., Stewart P.A., Welsh E.A., Boyle T.A., Meyer A.S., Koomen J.M, & Haura E.B. (2022). Integrated proteomics-based physical and functional mapping of AXL kinase signaling pathways and inhibitors define its role in cell migration. Molecular cancer research : MCR, 20(4), 542-555.

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Immunohistochemical Profiling of Liver Tissue

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The 5-μm liver sections (paraffin-embedded) were deparaffinized in xylene and dehydrated in graded alcohol series. Heat-induced antigen retrieval was performed in citrate buffer and endogenous peroxidase was blocked with 3% H₂O₂ solution. Slides were incubated with primary antibody (mouse anti-α-SMA: M0851, DAKO; rat anti-F4/80: sc-59171; Santa Cruz Biotechnology, Dallas, TX; rabbit anti-AXL: C89E7, Cell Signaling Technology, Danvers, MA) overnight in a wet chamber at 4°C. After rinsing with phosphate-buffered saline (PBS), the slides were incubated with a biotinylated antibody for 45 minutes in a wet chamber and developed with the ABC-HRP Kit (Vector Laboratories, Burlingame, CA) and peroxidase substrate DAB (Sigma-Aldrich). After rinsing the slides with tap water, they were counterstained with hematoxylin and mounted with Aquatex (Merck Millipore, Burlington, MA).

Tutusaus A., de Gregorio E., Cucarull B., Cristóbal H., Aresté C., Graupera I., Coll M., Colell A., Gausdal G., Lorens J.B., García de Frutos P., Morales A, & Marí M. (2019). A Functional Role of GAS6/TAM in Nonalcoholic Steatohepatitis Progression Implicates AXL as Therapeutic Target. Cellular and Molecular Gastroenterology and Hepatology, 9(3), 349-368.

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Immunofluorescence Imaging of MITF and Axl

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Melmet 1 and Melmet 5 cells grown on glass coverslips were fixed in 4% paraformaldehyde for 15 min on ice before staining with mouse anti-MITF (Thermo Scientific), or rabbit anti-Axl (Cell Signaling), both diluted 1 : 100 in PBS/0.05% saponin. After overnight incubation at 4 °C, the samples were stained for 1 h with donkey anti-mouse 488 or donkey anti-rabbit 549 (both Jackson ImmunoResearch) diluted 1 : 1500 or 1 : 400, respectively. The coverslips were mounted in Prolong Gold mounting medium (Life Technologies) containing DAPI and examined using a laser-scanning confocal microscope (LSM710; Carl Zeiss), equipped with a Plan-Apochromat ×63/1.4 Oil DICIII objective. Image processing and visualization were performed by using the ZEN Light 2011 software.

Eng M.S., Kaur J., Prasmickaite L., Engesæter B.Ø., Weyergang A., Skarpen E., Berg K., Rosenblum M.G., Mælandsmo G.M., Høgset A., Ferrone S, & Selbo P.K. (2018). Enhanced targeting of triple-negative breast carcinoma and malignant melanoma by photochemical internalization of CSPG4-targeting immunotoxins. Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology, 17(5), 539-551.

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