Alfuzosin hydrochloride (AFZ), ascorbic acid (AA), uric acid (UA), glucose (glu), cobalt nitrate hexahydrate (Co(NO3)2·6H2O), nickel nitrate hexahydrate (Ni(NO3)2·6H2O), 2-methylimidazole (2-MIM), potassium hydroxide (KOH), sodium chloride (NaCl), calciIum sulfate (CaSO4), iron(II) sulfate heptahydrate (FeSO4·7H2O), N,N-Dimethylformamide (DMF), human serum, sodium dihydrogen phosphate (NaH2PO4), and disodium hydrogen phosphate (NaH2PO4) were sourced from Sigma-Aldrich, United States. Methanol was procured from SK Chemicals, Republic of Korea. The tablet sample, ALFOO 10 mg, was purchased locally from pharmaceuticals in Asan, Republic of Korea. All compounds were used as received, without further purification.
The 0.05 M phosphate buffer saline (PBS) with a pH range of 5–9 was prepared by dissolving Na2HPO4 and NaH2PO4 in Millipore water and neutralizing it with 0.1 M HCl or NaOH. Millipore water was used to make all of the solutions.
The 0.05 M phosphate buffer saline (PBS) with a pH range of 5–9 was prepared by dissolving Na2HPO4 and NaH2PO4 in Millipore water and neutralizing it with 0.1 M HCl or NaOH. Millipore water was used to make all of the solutions.