UTD or CAR-expressing THP-1 cells were cocultured with GFP+ 293T cells or GFP+ 293T-S (S+) target cells for 4 h at 37°C. The effector-to-target (E:T) ratio was 1:1, and 1 × 105 cells were used as both effector cells and target cells. After coculturing, the cells were harvested and stained with an anti-CD11b APC-Cy7-conjugated antibody (M1/70, BioLegend) and analyzed by FACS using a FACSCalibur flow cytometer (BD Biosciences). The percentage of phagocytosis was calculated based on the percent of GFP+ events within the CD11b+ population. Data are represented as the mean ± standard error of quadruplicate wells.
Anti cd11b apc cy7 conjugated antibody
Manufactured by BioLegend
The Anti-CD11b APC-Cy7-conjugated antibody is a tool used for the identification and analysis of CD11b-expressing cells in flow cytometry experiments. CD11b is a cell surface integrin that is expressed on various immune cells, including monocytes, macrophages, and neutrophils. The APC-Cy7 fluorescent dye is conjugated to the antibody, allowing for its detection and quantification using flow cytometry instrumentation.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using anti cd11b apc cy7 conjugated antibody
1
Phagocytosis Assay with THP-1 Cells
2
Phagocytosis Assay with UTD or CAR-expressing THP-1 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
UTD or CAR-expressing THP-1 cells were cocultured with GFP + 293T cells or GFP + 293T-S (S + ) target cells for 4 h at 37 °C. The effector-to-target (E:T) ratio was 1:1, and 1 × ~ 9 ~ (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
The copyright holder for this preprint this version posted August 21, 2020. ; https://doi.org/10.1101/2020.07.26.222208 doi: bioRxiv preprint 10 5 cells were used as both effector cells and target cells. After coculturing, the cells were harvested and stained with an anti-CD11b APC-Cy7-conjugated antibody (M1/70, BioLegend) and analyzed by FACS using a FACSCalibur flow cytometer (BD Biosciences). The percentage of phagocytosis was calculated based on the percent of GFP + events within the CD11b + population. Data are represented as the mean ± standard error of quadruplicate wells.
The copyright holder for this preprint this version posted August 21, 2020. ; https://doi.org/10.1101/2020.07.26.222208 doi: bioRxiv preprint 10 5 cells were used as both effector cells and target cells. After coculturing, the cells were harvested and stained with an anti-CD11b APC-Cy7-conjugated antibody (M1/70, BioLegend) and analyzed by FACS using a FACSCalibur flow cytometer (BD Biosciences). The percentage of phagocytosis was calculated based on the percent of GFP + events within the CD11b + population. Data are represented as the mean ± standard error of quadruplicate wells.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!