αcd161 pe dx 12

Manufactured by BD

αCD161-PE (DX-12) is a fluorescently-labeled antibody that recognizes the CD161 antigen. CD161 is a C-type lectin-like receptor expressed on the surface of natural killer (NK) cells, a subset of T cells, and other immune cells. This product is intended for research use only.

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Lab products found in correlation

αcd196 ccr6 percp cy5.5 11a9, by BD (2 mentions) αcd4 fitc rpa t4, by BD (2 mentions) αcd183 cxcr3 apc 1 c6 cxcr3, by BD (2 mentions) Facsdiva v6, by BD (2 mentions) Facscanto 2, by BD (2 mentions) Foxp3 transcription factor staining buffer set, by Thermo Fisher Scientific (2 mentions) Rpa t4, by Thermo Fisher Scientific (1 mentions) αcd3 fitc, by BD (1 mentions) αcd19 apc, by BD (1 mentions) α cd8 apc cy7, by BD (1 mentions) Multitest 6 color tbnk, by BD (1 mentions) α cd4 pe cy7, by BD (1 mentions) Il 17, by BD (1 mentions)

2 protocols using αcd161 pe dx 12

Multiparametric Flow Cytometry of T-Cell Subsets in MS

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Whole blood cells of MS patients at baseline and after 14 days of PA supplementation were stained by BD Multitest™ 6-Color TBNK (αCD3-FITC; αCD16-PE+ αCD56-PE; αCD45-PerCP Cy5.5; αCD4-PE Cy7; αCD19-APC; αCD8-APC Cy7; BD). T-cell subsets were identified with αCD196 (CCR6)-PerCP Cy5.5 (11A9; BD), αCD4-FITC (RPA-T4; BD), αCD161-PE (DX-12; BD), and αCD183 (CXCR3)-APC (1 C6/CXCR3; BD) for determination of Th1 and Th17 as previously described.^{23 (link)} For Treg analysis, cells were stained with CD4-FITC (RPA-T4, eBioscience) and CD25-APC (BC96, eBioscience) and intracellular FoxP3-PE (236A/E7, eBioscience) by using Foxp3/Transcription Factor Staining Buffer Set (eBioscience) according to manufacturer protocol. Phenotyping was performed on BD FACS Canto2 and analyzed by BD FACS DIVA v6 and BD FACS Canto2 software.

Duscha A., Hegelmaier T., Dürholz K., Desel C., Gold R., Zaiss M.M, & Haghikia A. (2022). Propionic acid beneficially modifies osteoporosis biomarkers in patients with multiple sclerosis. Therapeutic Advances in Neurological Disorders, 15, 17562864221103935.

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Th17 and Treg Immunophenotyping Protocol

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Blood samples were analyzed before and after termination of PA intake. Th17 cells were identified with αCD196 (CCR6)-PerCP Cy5.5 (11A9; BD), αCD4-FITC (RPA-T4; BD), αCD161-PE (DX-12; BD), αCD183 (CXCR3)-APC (1C6/CXCR3; BD) and IL-17 (BD Bioscience) as previously described (16 (link)). For Treg analysis, cells were stained with αCD4-FITC (RPA-T4, eBioscience), αCD25-APC (BC96, eBioscience) and intracellular αFoxP3-PE (236A/E7, eBioscience) by using Foxp3/Transcription Factor Staining Buffer Set (eBioscience) according to manufacturer protocol. Phenotyping was performed on BD FACS Canto2 and analyzed by BD FACS DIVA v6 software.

Haase S., Mäurer J., Duscha A., Lee D.H., Balogh A., Gold R., Müller D.N., Haghikia A, & Linker R.A. (2021). Propionic Acid Rescues High-Fat Diet Enhanced Immunopathology in Autoimmunity via Effects on Th17 Responses. Frontiers in Immunology, 12, 701626.

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