Chns 932

Ruthenium(III) chloride hydrate, lithium chloride, 2,2 ′ -bipyridyl, silver tetrafluoroborate, triethylamine, 5-bromosalicylaldehyde, 3,5-dibromosalicylaldehyde, 5-chlorosalicylaldehyde, 3,5dichlorosalicylaldehyde, and 3-bromo-5-chlorosalicylaldehyde were purchased from Sigma-Aldrich and used without purification. Ru(bpy) 2 Cl 2 was prepared according to the literature procedure. [53] [54] [55] The commercial solvents were distilled and then used for the preparation of ligands and complexes. The FT-IR spectrum was recorded on a JASCO, FT/IR-6300 spectrometer (4000-400 cm -1 ) in KBr pellets. Elemental analysis was performed on Leco, CHNS-932 and PerkinElmer 7300 DV elemental analyzers. 1 H-and 13 C-NMR spectra for the Ru(II) complexes were recorded on a Bruker Avance III 400 spectrometer using DMSO as the solvent at 20 °C.

Human serum albumin (HSA) was purchased from Sigma-Aldrich. 2-Hydroxy-1-naphtaldehhyde and allylamine were obtained from Merck Co. and used without further purification. All salts used for buffer preparation were analytical grade and dissolved in double distilled water. All of the solutions were used freshly after preparation. The FT-IR spectra were recorded on a JASCO, FT/IR-6300 spectrometer (4000-400 cm -1 ) in KBr pellets. 1 H and 13 C NMR spectra were recorded on a Bruker Avance 400 spectrometer using DMSO as a solvent for the Palladium(II) complex. The elemental analysis was performed on Leco, CHNS-932 and Perkin-Elmer 7300 DV elemental analyzers. The UV-vis spectra were recorded on a JASCO V-670 spectrophotometer.
Fluorescence measurements were carried out on Shimadzu RF-5000 spectrofluorometer at room temperature. The circular dichroism (CD) spectra were recorded using Aviv spectropolarimeter model 215 (Proterion Corp., USA) at 25 °C. The scan speed was 20 nm min -1 . The CD spectra were recorded in the far-UV region (190-260 nm) using 0.1 cm path length cell.

All required materials were purchased from Sigma-Aldrich except 2-Hydroxy-1-naphtaldehhyde and (R/S)-1-aminopropan-2-ol that were obtained from Merck Co. The FT-IR spectrum was recorded on a JASCO, FT/IR-6300 spectrometer (4000-400 cm -1 ) in KBr pellets. The elemental analysis was performed on Leco, CHNS-932 and Perkin-Elmer 7300 DV elemental analyzers. The UV-Vis spectra were recorded on a JASCO V-670 spectrophotometer. Fluorescence measurements were carried out on Shimadzu RF-5000 spectrofluorometer at room temperature.
The circular dichroism (CD) spectra were recorded using Aviv spectropolarimeter model 215 (Proterion Corp., USA) at 25 °C.