Establishment of SSCs from Oct4-GFP/LacZ and Oct4-GFP transgenic mice (C57BL/6 background) was described previously (Ko et al. 2009 (link); Kanatsu-Shinohara et al. 2011 (link); Han et al. 2012 (link)). SSC medium for expansion was composed of StemPro-34 SFM (Gibco, Carlsbad, CA, USA) with the following supplements: StemPro (Gibco), 1× N2 (Gibco), 6 mg/ml d-(+)-glucose (Gibco), 30 mg/ml pyruvic acid (Gibco), 1 μl/ml DL-lactic acid (Sigma-Aldrich, Saint Louis, MO, USA), 5 mg/ml bovine serum albumin (BSA; Gibco), 1% fetal bovine serum (FBS; Gibco), 2 mM L-glutamine (Gibco), 50 μM β-mercaptoethanol (Gibco), 1×penicillin/streptomycin (Welgene, Gyeongsan, Korea), 1× minimal essential medium (MEM) with non-essential amino acids (Gibco), 1× MEM vitamins (Welgene), 30 ng/ml β-estradiol (Sigma), 60 ng/ml progesterone (Sigma), 20 ng/ml human EGF (Peprotech, Rocky Hill, NJ, USA), 20 ng/ml human bFGF (Peprotech), 20 ng/ml human GDNF (Peprotech), and 103 U/ml murine leukemia inhibitory factor (Prospec, Rehovot, Israel).
Mem vitamins
Manufactured by Welgene
MEM vitamins is a laboratory product that provides a balanced mixture of essential vitamins required for cell culture media. It is designed to supplement and support the growth and maintenance of various cell lines in in vitro applications.
Automatically generated - may contain errors
Lab products found in correlation
Pyruvic acid, by Thermo Fisher Scientific (2 mentions)
Progesterone, by Merck Group (2 mentions)
Human bfgf, by Thermo Fisher Scientific (2 mentions)
Murine leukemia inhibitory factor, by ProSpec (2 mentions)
Dl lactic acid, by Merck Group (2 mentions)
D glucose, by Thermo Fisher Scientific (2 mentions)
β estradiol, by Merck Group (2 mentions)
Human egf, by Thermo Fisher Scientific (2 mentions)
Human gdnf, by Thermo Fisher Scientific (2 mentions)
Bovine serum albumin (bsa), by Thermo Fisher Scientific (2 mentions)
L glutamine, by Thermo Fisher Scientific (2 mentions)
Stempro 34 sfm, by Thermo Fisher Scientific (2 mentions)
β mercaptoethanol, by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin, by Welgene (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Stempro, by Thermo Fisher Scientific (1 mentions)
Non essential amino acids (neaa), by Thermo Fisher Scientific (1 mentions)
Stempro supplement, by Thermo Fisher Scientific (1 mentions)
N2 supplement, by Thermo Fisher Scientific (1 mentions)
Mem non essential amino acid, by Thermo Fisher Scientific (1 mentions)
2 protocols using mem vitamins
1
Establishment of Transgenic SSCs from Mice
2
Establishment of SSCs from Oct4-GFP/LacZ Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Establishment of SSCs from Oct4-GFP/LacZ transgenic mice (C57BL/6 background) was described previously (Ko et al., 2009 (link); 2010 (link); 2012 (link)). SSC medium for expansion was composed of StemPro-34 SFM (Gibco) with the following supplements: StemPro supplement (Gibco), 1× N2 supplement (Gibco), 6 mg/ml d-(+)-glucose (Gibco), 30 mg/ml pyruvic acid (Gibco), 1 μl/ml DL-lactic acid (Sigma-Aldrich), 5 mg/ml bovine serum albumin (BSA; Gibco), 1% fetal bovine serum (Gibco), 2 mM L-glutamine (Gibco), 50 μM β-mercaptoethanol (Gibco), 1×penicillin/streptomycin (Welgene), 1× minimal essential medium (MEM) non-essential amino acids (Gibco), 1× MEM vitamins (Welgene), 30 ng/ml β-estradiol (Sigma-Aldrich), 60 ng/ml progesterone (Sigma-Aldrich), 20 ng/ml human EGF (Peprotech), 20 ng/ml human bFGF (Peprotech), 20 ng/ml human GDNF (Peprotech), and 103 U/ml murine leukemia inhibitory factor (Prospec).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!