BM-hMSCs were purchased from the American Type Culture Collection, according to previous cell culture methods [36] . Rhynchophylline (Methyl (7b, 16E, 20a)-16-(methoxymethylene)-2-oxocorynoxan-17oate) was obtained from Toronto Research Chemical (R414180, Toronto, Ontario, Canada). BM-hMSCs were suspended in low-glucose DMEM (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (Thermo Fisher Scientific) in the absence of antibiotics. The cells were plated at a density of 1.2 £ 10 4 cells/cm 2 in poly-l-lysineÀcoated 96-well or '10-cm culture plate at 37°C in a humidified atmosphere containing 95% O 2 and 5% CO 2 (Figure 1).
Bm hmscs
Manufactured by Thermo Fisher Scientific
Sourced in United States
BM-hMSCs are human mesenchymal stem cells derived from bone marrow. They are characterized by their ability to differentiate into various cell lineages, including osteoblasts, chondrocytes, and adipocytes. The core function of BM-hMSCs is to serve as a model system for studying stem cell biology, tissue regeneration, and disease mechanisms.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Low glucose dmem, by Thermo Fisher Scientific (1 mentions)
Hek293 cell, by Thermo Fisher Scientific (1 mentions)
Hucct 1, by Nacalai Tesque (1 mentions)
Penicillin streptomycin, by Nacalai Tesque (1 mentions)
Hek293 cell, by American Type Culture Collection (1 mentions)
Rpmi 1640 medium, by Nacalai Tesque (1 mentions)
Bm hmscs, by Lonza (1 mentions)
Hpbmc, by Lonza (1 mentions)
2 protocols using bm hmscs
1
Rhynchophylline Effects on BM-hMSCs
2
Cell Culture Conditions of Common Cell Lines
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HEK293 cells were purchased from the American Type Culture Collection (Manassas, VA, USA). HuCCT1 and HEL cells were purchased from the Japanese Cancer Research Resources Bank (Japan). BM-hMSCs and hPBMCs were purchased from Lonza Group AG (Switzerland). HEK293 cells were maintained in Dulbecco’s modified Eagle medium (DMEM) high-glucose GlutaMAX supplement (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS; Thermo Fisher Scientific) and 1% penicillin-streptomycin (Nacalai Tesque). HuCCT1 and HEL cells were maintained in RPMI 1640 medium (Nacalai Tesque) supplemented with 10% FBS and 1% penicillin-streptomycin. BM-hMSCs were maintained in DMEM (Thermo Fisher Scientific) supplemented with 10% FBS and penicillin-streptomycin.
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