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Macs magnetic enrichment column

Manufactured by Miltenyi Biotec
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The MACS magnetic enrichment column is a laboratory equipment designed to facilitate the magnetic separation and isolation of target cells or molecules from complex biological samples. The column utilizes a strong magnetic field to capture and retain magnetically labeled cells or particles, allowing for their efficient separation and purification. This core function is achieved through the column's specialized design and construction, which enables the effective magnetic enrichment of the desired samples.

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Lab products found in correlation

Anti human cd34 antibody, by BD (2 mentions) Human fc block, by Miltenyi Biotec (2 mentions)

2 protocols using macs magnetic enrichment column

1

Isolation of Hematopoietic Stem and Progenitor Cells

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HSPCs (CD34+ cells) were isolated from cryopreserved fetal or juvenile bone marrow MNCs or fetal liver MNCs via a MACS magnetic enrichment column (Miltenyi, Gaithersburg, MD). At least 10,000,000 viable bone marrow MNCs or 2,000,000 liver MNCs were incubated with anti-human CD34 antibody (BD Biosciences, clone 563) and human Fc block (Miltenyi) together using 3 μL and 6 μL, respectively, per 1,000,000 cells for 20 min at room temperature. The rest of the MACS column protocol was followed according to manufacturer’s instructions (Miltenyi). The CD34-flow-through fraction was collected in addition to the CD34+ fraction. CD34 enrichment efficiency was validated with flow cytometry. Enriched fractions yielded over 90% HSPCs.
Nash M.J., Dobrinskikh E., Soderborg T.K., Janssen R.C., Takahashi D.L., Dean T.A., Varlamov O., Hennebold J.D., Gannon M., Aagaard K.M., McCurdy C.E., Kievit P., Bergman B.C., Jones K.L., Pietras E.M., Wesolowski S.R, & Friedman J.E. (2023). Maternal diet alters long-term innate immune cell memory in fetal and juvenile hematopoietic stem and progenitor cells in nonhuman primate offspring. Cell reports, 42(4), 112393.
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2

Isolation of Hematopoietic Stem and Progenitor Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
HSPCs (CD34+ cells) were isolated from cryopreserved fetal or juvenile bone marrow MNCs or fetal liver MNCs via a MACS magnetic enrichment column (Miltenyi, Gaithersburg, MD). At least 10,000,000 viable bone marrow MNCs or 2,000,000 liver MNCs were incubated with anti-human CD34 antibody (BD Biosciences, clone 563) and human Fc block (Miltenyi) together using 3 μL and 6 μL, respectively, per 1,000,000 cells for 20 min at room temperature. The rest of the MACS column protocol was followed according to manufacturer’s instructions (Miltenyi). The CD34-flow-through fraction was collected in addition to the CD34+ fraction. CD34 enrichment efficiency was validated with flow cytometry. Enriched fractions yielded over 90% HSPCs.
Nash M.J., Dobrinskikh E., Soderborg T.K., Janssen R.C., Takahashi D.L., Dean T.A., Varlamov O., Hennebold J.D., Gannon M., Aagaard K.M., McCurdy C.E., Kievit P., Bergman B.C., Jones K.L., Pietras E.M., Wesolowski S.R, & Friedman J.E. (2023). Maternal diet alters long-term innate immune cell memory in fetal and juvenile hematopoietic stem and progenitor cells in nonhuman primate offspring. Cell reports, 42(4), 112393.
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