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2 protocols using sodium tricitrate

1

HPLC-Based Amino Acid Analysis Protocol

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2-Chlorophenylalanine, HPLC-grade acetonitrile, acetic acid, ethanol, methanol, acetone, and formic acid (FA); Tris, sodium dodecyl sulfate, hydrochloric acid, dithiothreitol (DTT), iodoacetamide (IAA), and ammonium bicarbonate were purchased from Sinopharm Chemical Reagent Co. Ltd., Shanghai, China. Sodium tricitrate, dl-dithiotriol, urea, sodium dihydrogen phosphate, hydroxypropyl methylcellulose were purchased from Sigma-Aldrich company, USA. Bicinchoninic acid (BCA) assay were purchased from Beyotime Biotechnology, Shanghai, China. Trypsin (TPCK-treated) was purchased from Thermo Fisher Scientific (Rockford, IL, USA). Pure water for the experiment was prepared with ultrapure water (18.2 MΩ·cm, 25 °C).
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2

Synthesis and Functionalization of Gold Nanoparticles

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All chemicals were of analytical grade and used without any further purification. Aniline, hexaammonium heptamolybdate tetrahydrate [(NH 4 ) 6 Mo 7 O 24 •4H 2 O], HAuCl 4 , thiourea, and ethanol were purchased from Thermofisher Scientific Inc (Waltham, MA, USA). Ammonium persulfate (NH 4 ) 2 S 2 O 8 , sodium tricitrate (Na 3 C 6 H 5 O 7 ), [Fe(CN) 6 ] 3-/4-, KCl, N-hydroxysuccinimide (NHS), bovine serum albumin (BSA), mercaptopropionic acid (MPA), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC), 0.01 M phosphate-buffered saline (PBS) buffer powder, and 100 mM PBS buffer were purchased from Sigma-Aldrich Co. Anti-E. coli antibody was purchased from Abcam plc. A Milli-Q system (Millipore, Merck KGaA, Darmstadt, Germany) supplied deionized (DI) water with a resistivity of 18.2 MΩ•cm at 25 • C throughout the experiments.
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