Fetal bovine serum (FBS): Millipore (Billerica, MA, USA); High-glucose DMEM medium: Hyclone (Logan, Utah, USA). Rabbit anti-C-Myc, anti-Cyclin D1, anti-β-catenin and APC monoclonal antibodies: Cell Signaling (Danvers, MA, USA); Rabbit anti-MMP-9 antibody: Millipore (Billerica, MA, USA); Rabbit anti-ZEB1 antibody: Abcam (Eugene, USA); anti-MMP-3 and anti-β-actin monoclonal antibodies: Bioworld (Shanghai, China).
Animal experiments 20g nude mice were subcutaneously injected with 5×10 6 SGC-7901/DDP cells with miR-200b mimics or NC lentivirus. After 2 weeks, the tumor size wad observed and measured. At the 3rd week, the mice were sacri ced by neck dragging. Tumors were photographed and weighed. All experimental protocols used on the animals were approved by the institutions' subcommittees on animal care of Anhui Medical University.
Animal experiments 20g nude mice were subcutaneously injected with 5×10 6 SGC-7901/DDP cells with miR-200b mimics or NC lentivirus. After 2 weeks, the tumor size wad observed and measured. At the 3rd week, the mice were sacri ced by neck dragging. Tumors were photographed and weighed. All experimental protocols used on the animals were approved by the institutions' subcommittees on animal care of Anhui Medical University.