The antibodies used in this study were as follows: HRP-anti-hemagglutinin (12013819001) and anti-c-Myc-HRP (11814150001) (Roche Applied Science); HRP-anti-Flag (M2) (A8592) and anti-β-actin (A1978) (Sigma-Aldrich, St. Louis, MO); anti-phospho-IKKα/β (No. 2697s), anti-phospho-JNK (No.9251), anti-JNK (No. 9252) anti-phospho-ERK (No. 9101), anti-ERK (No. 9102), anti-phospho-ERK (No. 9101), anti-ERK (No. 9102), anti-phospho-p38 (No. 9211), anti-p38 (No. 9212), anti-p65/RelA (No. 6956), anti-IκBα (No. 4814) and anti-phospho-IkBα (Ser32/36) (No. 9246) (Cell Signaling Technology, Danvers, MA)); anti-IKK (Merck Millipore, Billerica, MA); Anti-LRRC25 (ab84954) (Abcam, Cambridge, U.K.); Mouse mAb HA tag DyLight 650(ab117515) (Abcam, Cambridge, U.K.). Recombinant human TNF-α was purchased from PeproTech; Lipopolysaccharides (LPS) (L4391-1 MG) and Bafilomycin A1 were purchased from Sigma-Aldrich (St. Louis, MO). Trizol was purchased from Invitrogen and Transcript First Strand cDNA Synthesis Kit from Roche.
Hrp anti hemagglutinin
Manufactured by Roche
Sourced in Switzerland
The HRP-anti-hemagglutinin is a laboratory reagent used for the detection and quantification of hemagglutinin, a protein found on the surface of influenza viruses. This product is designed to be used in various immunoassay techniques, such as enzyme-linked immunosorbent assay (ELISA), to help researchers and scientists study influenza virus biology and develop potential treatments or vaccines.
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Lab products found in correlation
Anti β actin, by Merck Group (1 mentions)
Anti p65 rela, by Cell Signaling Technology (1 mentions)
Anti erk, by Cell Signaling Technology (1 mentions)
Anti phospho erk, by Cell Signaling Technology (1 mentions)
Trizol, by Thermo Fisher Scientific (1 mentions)
Bafilomycin a1, by Merck Group (1 mentions)
Transcript first strand cdna synthesis kit, by Roche (1 mentions)
Anti phospho ikbα ser32 36, by Cell Signaling Technology (1 mentions)
Anti p38, by Cell Signaling Technology (1 mentions)
Recombinant human tnf α, by Thermo Fisher Scientific (1 mentions)
Anti flag m2 hrp, by Merck Group (1 mentions)
Lipopolysaccharides lps l4391 1mg, by Merck Group (1 mentions)
Anti c myc hrp, by Roche (1 mentions)
Anti phospho jnk, by Cell Signaling Technology (1 mentions)
Anti jnk, by Cell Signaling Technology (1 mentions)
Anti phospho p38, by Cell Signaling Technology (1 mentions)
Anti iκbα, by Cell Signaling Technology (1 mentions)
Anti stub1 12496 r034, by Sino Biological (1 mentions)
Anti β tubulin hc101, by Transgene (1 mentions)
Anti mouse igg hrp ap308p, by Merck Group (1 mentions)
2 protocols using hrp anti hemagglutinin
1
Antibody Characterization and Analysis
2
Wnt-3a Signaling Pathway Regulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Recombinant Human Wnt-3a (NP-149122) was purchased from an R&D system (Minneapolis, MN, USA).
Horseradish peroxidase (HRP)-anti-Flag (M2) (A8592), anti-β-actin (A1978), anti-rabbit-IgG-HRP (AP132P), and anti-mouse-IgG-HRP (AP308P) were purchased from Sigma (St. Louis, MO, USA). HRP-anti-hemagglutinin (12013819001) was purchased from Roche Applied Science (Switzerland, Basel). anti-c-Myc (HT101) and anti-β-tubulin (HC101) were purchased from TransGen Biotech (Beijing, China). anti-β-Catenin (8480) and anti-non-phospho β-Catenin (19807) were purchased from Cell Signaling Technology (Danvers, MA, USA). anti-LMNB1 (101237-T32) and anti-STUB1 (12496-R034) were purchased from Sino Biological (Beijing, China). anti-LEF1 (sc-374522) and anti-Arc (sc-17839) were purchased from Santa Cruz Biotechnology (San Diego, CA, USA).
Empty vector pcDNA3.1 was kindly provided by Dr Jun Cui (Sun Yat-sen University, Guangzhou, China). Target genes were cloned from A549 cDNA and subcloned into the pcDNA3.1 vector. The plasmids were confirmed by DNA sequencing at Sangon Biotech (Shanghai, China).
Horseradish peroxidase (HRP)-anti-Flag (M2) (A8592), anti-β-actin (A1978), anti-rabbit-IgG-HRP (AP132P), and anti-mouse-IgG-HRP (AP308P) were purchased from Sigma (St. Louis, MO, USA). HRP-anti-hemagglutinin (12013819001) was purchased from Roche Applied Science (Switzerland, Basel). anti-c-Myc (HT101) and anti-β-tubulin (HC101) were purchased from TransGen Biotech (Beijing, China). anti-β-Catenin (8480) and anti-non-phospho β-Catenin (19807) were purchased from Cell Signaling Technology (Danvers, MA, USA). anti-LMNB1 (101237-T32) and anti-STUB1 (12496-R034) were purchased from Sino Biological (Beijing, China). anti-LEF1 (sc-374522) and anti-Arc (sc-17839) were purchased from Santa Cruz Biotechnology (San Diego, CA, USA).
Empty vector pcDNA3.1 was kindly provided by Dr Jun Cui (Sun Yat-sen University, Guangzhou, China). Target genes were cloned from A549 cDNA and subcloned into the pcDNA3.1 vector. The plasmids were confirmed by DNA sequencing at Sangon Biotech (Shanghai, China).
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