Cardiomyocyte sarcomere contraction properties were evaluated using a high speed NTSC camera (MyoCamCCD100V, Ionoptix, Milton, MA, USA) through a fast Fourier transform for sarcomere deconvolution-based analysis (IonWizard, Ionoptix, Milton, MA, USA). During this experiments, freshly isolated left ventricle cardiomyocytes were placed in a coverslip bathed with Tyrode solution and assembled into a chamber containing a pair of platinum electrodes from which cells were field stimulated (MyoPacer, IonOptix, Milton, MA, USA) with 4 ms duration and 60 V amplitude biphasic pulses, at stimulation frequency of 1 Hz. Signal was collected at 250 Hz rate. Five consecutive events of sarcomere shortening and re-lengthening were averaged for each cell analysis. The occurrence of extra contractions was evaluated through 60 s sarcomere detected contractions using the same stimulation protocol. The resting sarcomere length was measured using a fast Fourier transform algorithm (IonWizard, Ionoptix, Milton, MA, USA), in a relaxed state, without stimulation. All experiments were performed at room temperature (22–25 °C).
Myocamccd100v
Manufactured by IonOptix
Sourced in United States
The MyoCamCCD100V is a high-speed, low-noise CCD camera designed for live cell imaging applications. It features a 1004 x 1002 pixel CCD sensor with a pixel size of 8 x 8 μm, allowing for high-resolution imaging. The camera can capture images at a frame rate of up to 100 frames per second, making it suitable for applications that require rapid data acquisition. The MyoCamCCD100V is designed to interface with a variety of microscope systems and software platforms, providing a versatile solution for researchers and scientists.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using myocamccd100v
1
Cardiomyocyte Sarcomere Contraction Analysis
2
Measuring Cardiomyocyte Contractility
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mouse cardiomyocytes were placed in a chamber with a glass coverslip base mounted on the stage of an inverted microscope (TCM 400). Cells were stimulated by using platinum bath electrodes with voltage pulses (40V intensity during 5 ms, 1 Hz frequency). The cardiomyocytes were visualized on a PC monitor with a NTSC camera (MyoCamCCD100V; Ionoptix) and the images collected were used to measure cell shortening in response to electrical stimulation, using a video motion edge detection system (Ionoptix). The cell image was sampled at 240 Hz. Cell shortening was calculated from the output of the edge detector using an IonWizard A/D converter (Ionoptix) (Figure 1c).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!