Human embryonic kidney cells (HEK293T) and human lung cancer cells (A549) were purchased from NCCS, Pune and cultured in Dulbecco’s Modified Eagle’s Medium (Himedia). Human splenic marginal zone lymphoma SSK-41 cell line was established and cultured in RPMI 1640 medium (Himedia) and B-cell lymphoma cells (JM1) was purchased from NCCS, Pune and cultured in Iscove’s Modified Dulbecco’s Medium (Himedia) supplemented with 10% fetal bovine serum (Gibco, Thermo fisher Scientific, Waltham, MA, USA) and 1% penicillin/streptomycin solution in a humidified incubator at 37°C and a 5% of CO2 atmosphere [41 (link)].
Iscove s modified dulbecco s medium
Manufactured by HiMedia
Sourced in United States, India
Iscove's Modified Dulbecco's Medium (IMDM) is a cell culture medium that provides a balanced salt solution and essential nutrients for the growth and maintenance of a variety of cell types. It is designed to support the growth of hematopoietic cells and other sensitive cell lines.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 medium, by HiMedia (1 mentions)
Dulbecco s modified eagle s medium, by HiMedia (1 mentions)
Fetal bovine serum (fbs), by HiMedia (1 mentions)
Potato dextrose agar (pda), by HiMedia (1 mentions)
Phosphate buffer saline (pbs), by HiMedia (1 mentions)
Trypsin edta, by HiMedia (1 mentions)
Chloramphenicol, by HiMedia (1 mentions)
2 protocols using iscove s modified dulbecco s medium
1
Cell Culture Protocols for Cancer Research
2
Antimicrobial Assay Protocol
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Potato dextrose agar (PDA), Modified Bold's Basal Salt medium (MBBS), Malt Yeast Extract agar (MYE), spot test reagents (KOH), calcium hypochlorite (C test reagent), phenylene diamine (PD test reagent), Silica gel (230-400 mesh), Standard solution as Chloramphenicol (25mg/mL distilled water) and Fluconazole (25mg/ mL distilled water-30μl), 3-(4,5-dimethylthiozol-2-yl)-3,5-diphenyl tetrazolium bromide (MTT), Iscove's Modified Dulbecco's Medium (IMDM), trypsin-EDTA, Fetal Bovine Serum (FBS) and Phosphate Buffer Saline (PBS) were purchased from Himedia laboratories, Mumbai, India. Hexane, benzene, ethyl acetate, sulphuric acid, methanol and other solvents were purchased from SRL Ltd, Mumbai. All the chemicals purchased were of analytical grade with high purity.
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