Doxorubicin hydrochloride salt dox hcl

Vinylbenzyl chloride, 4,4'-Dithiodibutyric acid, oligo(ethylene glycol) methacrylate (OEG950 monomer, average M_n=950), 4-Cyano-4-(phenylcarbonothioylthio)pentanoic acid, 2, 2-Azobis(isobutyronitrile) (AIBN), trypsin-EDTA solution, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) and Dulbecco's Modified Eagle's Medium (DMEM) were all bought from Sigma-Aldrich (MO, U. S. A.). AIBN was purified by recrystallization in anhydrous ethanol. 1-hydroxybenzotriazole (HOBT) and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide HCl (EDC) were purchased from GL Biochem (Shanghai, China). Diisopropylethylamine (DIPEA) was purchased from Acros Organics. Paclitaxel was purchased from AK Scientific Inc. (CA, U. S. A.). Doxorubicin hydrochloride salt (DOX·HCl) and gemcitabine (GEM) were purchased from LC Laboratories (MA, USA). Fetal bovine serum (FBS) and penicillin-streptomycin solution were purchased from Invitrogen (NY, U. S. A.). The animal-related experiments were performed in full compliance with institutional guidelines and approved by the Animal Use and Care Administrative Advisory Committee at the University of Pittsburgh. Informed consent was obtained for experimentation with patient-derived xenograft (PDX) model. And the PDX-related experiments were carried out in accordance with The Code of Ethics of the World Medical Association.