Cellulose acetate ca

In the study, quercetin (QUE, 95%; Sigma-Aldrich, St. Louis, MO, USA), rutin (RUT, 97+%; Acros Organics, Geel, Belgium), polyethylene glycol (PEG; Fluka, Buchs, Switzerland), cellulose acetate (CA; Aldrich, St. Louis, MO, USA) with = 30,000 g/mol, and Tween 80 (Acros Organics, Geel, Belgium) were used. Both acetone and ethanol were of analytical grade (Sigma-Aldrich, Darmstadt, Germany) and were used as supplied. 2,2-Diphenyl-1-picrylhydrazyl (DPPH) from Sigma-Aldrich (Darmstadt, Germany), 3-(4.5-dimethylthiazol-2-yl)-2.5-diphenyltetrazolium bromide (MTT; Sigma-Aldrich, Darmstadt, Germany), ethidium bromide (EtBr; Sigma Chemical, Balcatta, WA, Australia), acridine orange (AO; Sigma Chemical, Balcatta, WA, Australia), and 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI; Sigma-Aldrich Darmstadt, Germany) were utilized without additional purification as they were of analytical grade of purity. The antibiotics penicillin and streptomycin (Lonza, Cologne, Germany) and fetal calf serum (FCS; Gibco, Wien, Austria) were added to Dulbecco’s Modified Eagle’s Medium (DMEM; Sigma-Aldrich, Darmstadt, Germany).

Cellulose acetate (CA, Aldrich, St. Louis, MO, USA) with ${\overline{\mathrm{M}}}_{\mathrm{n}}$ at 30,000 g/mol and 39.8 wt% of degree of substitution in acetyl content, polyethylene glycol (PEG, Fluka, Buchs, Switzerland) with relative molecular mass (Mr) 1900–2000 g/mol, polyethylene oxide (PEO, Serva, Heidelberg, Germany) with Mr ca. 100,000 g/mol and 5-chloro-8-hydroxyquinolinol (5-Cl8Q, Sigma-Aldrich, Buchs, Switzerland) were used. Acetone (Sigma-Aldrich) of analytical grade of purity was used. Potato dextrose agar medium was purchased from Merck, Darmstadt, Germany. The disposable consumables were supplied by Orange Scientific, Braine-l’Alleud, Belgium.

Cellulose acetate (CA, Aldrich, St. Louis, MO, USA) with a ${\overline{\mathrm{M}}}_{\mathrm{n}}$ of 30,000 g/mol and DS of 39.8%, polyethylene glycol (PEG, Fluka, Buchs, Switzerland) with Mr = 1900–2200 g/mol, and 5-chloro-8-hydroxyquinoline (5-Cl8Q, Sigma-Aldrich, Buchs, Switzerland) were used. Acetone (Sigma-Aldrich, Darmstadt, Germany) of analytical-grade purity was used. Dulbecco’s modified Eagle’s medium (DMEM) (Sigma-Aldrich, Darmstadt, Germany), enriched with fetal calf serum (FCS) (Gibco, Wien, Austria) and containing antibiotics (100 U/mL of penicillin, 0.1 mg/mL of streptomycin, LONZA, Cologne, Germany), was used. Trypsin–EDTA was supplied by FlowLab, Australia. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), ethidium bromide (EtBr), acridine orange (AO), and 4′,6-diamidino-2-phenylindole (DAPI) were purchased from Sigma–Aldrich, Darmstadt, Germany. The disposable consumables were supplied by Orange Scientific, Braine-l’Alleud, Belgium. HeLa human cervical cancer cells (ATCC, CCL-2), SH-4 (Homo sapiens skin melanoma) (ATCC, Rockville, MD, USA), and mouse BALB/3T3 clone A31 cell line (ATCC, CCL-163) were obtained from the American Type Cultures Collection (ATCC, Rockville, MD, USA). All chemicals used were of analytical grade and were used as received without any further purification.