cDNA samples (1 μl) were distributed in the RT-qPCR MicroAmp Fast Optical 96-well reaction plates [TermoFisher] and combined with 0.75 μl Taqman gene expression assay [Applied Biosystems], 12.5 μl TaqMan Fast Advanced Master Mix [Applied Biosystems], and 9.25 μl DNA/RNA/RNase free water, following the Taqman gene expression assay protocol [Thermofisher]. RT-PCR was performed in 7500 Fast Real-Time PCR System [Applied Biosystems] according to the Applied Biosystems protocol (holding stage of 10 min at 95 °C, following 40 cycles of 10 s at 95 °C and 30 s at 60 °C). The relative gene expression was normalized to the housekeeping gene ß-Actin. Primers are listed in Table 1 .
Rt qpcr microamp fast optical 96 well reaction plates
Manufactured by Thermo Fisher Scientific
The RT-qPCR MicroAmp Fast Optical 96-well reaction plates are designed for use in real-time quantitative PCR (RT-qPCR) applications. These plates provide a standardized format for conducting RT-qPCR experiments with high optical clarity and compatibility with various real-time PCR instruments.
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Lab products found in correlation
2 protocols using rt qpcr microamp fast optical 96 well reaction plates
1
RT-qPCR Gene Expression Profiling Protocol
2
RT-qPCR Gene Expression Profiling Protocol
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cDNA samples (1 μl) were distributed in the RT-qPCR MicroAmp Fast Optical 96-well reaction plates [TermoFisher] and combined with 0.75 μl Taqman gene expression assay [Applied Biosystems], 12.5 μl TaqMan Fast Advanced Master Mix [Applied Biosystems], and 9.25 μl DNA/RNA/RNase free water, following the Taqman gene expression assay protocol [Thermofisher]. RT-PCR was performed in 7500 Fast Real-Time PCR System [Applied Biosystems] according to the Applied Biosystems protocol (holding stage of 10 min at 95 °C, following 40 cycles of 10 s at 95 °C and 30 s at 60 °C). The relative gene expression was normalized to the housekeeping gene ß-Actin. Primers are listed in Table 1 .
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