Human liver cancer cell lines SNU-449 and Hep G2 were obtained from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). All cancer cells were cultured following the American Type Culture Collection (ATCC) instructions. The proliferation rates of two cell lines were measured using Cell Counting Kit-8 (CCK-8) (Dojindo, Mashiki, Japan). Briefly, 2000 cells per well were seeded in 96-well plates (Corning, New York, NY, USA.). After incubation for 24 h, four metabolic inhibitors, purchased from Selleckchem, were added to each well. After 48 h, CCK-8 solution was added to the cells, and the optical density (OD) values were measured at 450 nm after 60 min incubation.
Snu 449
Manufactured by National Collection of Authenticated Cell Cultures
Sourced in China
The SNU-449 is a cell line that was derived from a human hepatocellular carcinoma. It is a valuable tool for research and study in the field of liver cancer.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (4 mentions)
Hepg2, by National Collection of Authenticated Cell Cultures (3 mentions)
Snu449, by Thermo Fisher Scientific (3 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Hep3b, by Thermo Fisher Scientific (2 mentions)
Hep3b, by National Collection of Authenticated Cell Cultures (2 mentions)
Mhcc 97h, by National Collection of Authenticated Cell Cultures (2 mentions)
Hcclm3, by National Collection of Authenticated Cell Cultures (2 mentions)
Rpmi 1640, by Thermo Fisher Scientific (2 mentions)
Cell counting kit 8 cck 8, by Dojindo Laboratories (1 mentions)
96 well plate, by Corning (1 mentions)
Ab8978, by Abcam (1 mentions)
Ab40772, by Abcam (1 mentions)
Ab126772, by Abcam (1 mentions)
Ab50581, by Abcam (1 mentions)
Ab6721, by Abcam (1 mentions)
Snu 387, by Thermo Fisher Scientific (1 mentions)
Complete rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Ab8227, by Abcam (1 mentions)
Snu387, by National Collection of Authenticated Cell Cultures (1 mentions)
6 protocols using snu 449
1
Cytotoxicity Evaluation of Liver Cancer Cell Lines
2
Comparing Human Liver Cancer Cell Lines
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Five human HCC cell lines (SNU387, Huh7, Hep3B, and SNU449) were purchased from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). Huh7 cells were grown in Dulbecco's modified Eagle's medium (Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA), Hep3B cells were cultured in minimum essential medium, and SNU387, SNU449 cells were incubated in RPMI-1640 complete medium (Gibco; Thermo Fisher Scientific, Inc.). All cell media were supplemented with 10% fetal bovine serum (Gibco; Thermo Fisher Scientific, Inc.) and 100 U/ml streptomycin and penicillin. All cell lines were incubated at 37°C in 5% CO2. DOX and DAPI were purchased from Sigma-Aldrich; Merck KGaA (Darmstadt, Germany). The following antibodies were used: FSCN-1 (1:1,000, ab126772), while antibodies against β-actin (1:1,000, ab8227), Twist (1:1,000, ab50581), E-cadherin (1:1,000, ab40772), Vimentin (1:1,000, ab8978) and horseradish peroxidase (HRP)-conjugated goat anti-rabbit secondary antibodies (1:2,000, ab6721), all of which were purchased from Abcam (Cambridge, MA, USA), whereas HRP-conjugated goat anti-mouse secondary antibodies (1:2,000, 7076) were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA).
3
Cultivation of Human Liver Cancer Cell Lines
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Human HCC cell lines, including HCCLM3, HepG2, Huh-7, MHCC-97H, MHCC-97 L, SK-Hep1, SMMC-7721, SNU-423 and SNU-449 were purchased from Cell Bank of the Chinese Academy of Sciences. Dulbecco’s modified Eagle’s medium (DMEM) contained 10% (v/v) fetal calf serum (FCS) and 1% antibiotics was used. Cells were incubated at 37 °C in a humidified incubator under 5% CO2 condition.
4
Validation and Cultivation of Hepatocarcinoma Cell Lines
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Human hepatocarcinoma cell lines, including SUN387, SNU449, HepG2, Li-7, and Huh7, were obtained from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). Hep3B, PLC/PRF/5, and SMMC-7721 were purchased from ATCC (Manassas, VA, USA). Short Tandem Repeat profiling was used to validate the cell lines, and PCR was used to determine that they were mycoplasma-free. SNU449 and PLC/PRF/5 were grown in RPMI 1640 (Gibco BRL, USA), whereas the remaining cell lines were cultured in DMEM medium (Gibco BRL, USA). All the culture medium was supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin, and the plates were maintained at 37 °C in a humidified atmosphere of 5% CO2. The cells were passaged at a 1:3 split ratio when they reached 85–90% confluence.
5
Hepatocellular Carcinoma Cell Lines
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HCC cell lines SNU449 (RRID: CVCL_0454) and LM3 (RRID: CVCL_6832) were purchased from the Cell Bank of Chinese Academy of Sciences (Shanghai, China), and Hepa1–6 cell line was purchased from the American Type Culture Collection (ATCC, USA, RRID: CVCL_0327). Huh7 (RRID: CVCL_0336) and Huh7-derived RAPA resistant cells were kind gifts from Xiao Xu lab of Zhejiang University. All cell lines were authenticated by Short Tandem Repeat (STR) tests. Recent mycoplasma testing has been performed. Cells were cultured in DMEM (Thermo, USA) supplemented with 10% fetal bovine serum (FBS, Gibco, Australia) and further maintained at 37 °C under a humidified 5% CO2 condition.
6
Cell Culture Protocols for Liver Cancer
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HCC cell lines (MHCC-97H, SNU-449, Huh7, Hep3B, BEL-7405, and HCC-LM3) and normal liver cells (THLE-2) were purchased from the Cell Bank of Chinese Academy of Sciences (shanghai, China). DMEM (Thermo Fisher Scientific, South America) was used as a culture medium for Huh7, HCC-LM3, Hep3B, and MHCC-97H cells, whereas RPMI-1640 from the same company was used for BEL-7405, SNU-449 and THLE-2 culture. These media contained 10% FBS from the same company. Cells were cultured in a 37°C incubator under 5% CO2/95% atmosphere. Unless otherwise stated, all the chemicals and relevant reagents were purchased from Sigma-Aldrich (Saint Louis, MO, United States).
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