Protein adsorption onto the films was determined to examine whether nano coating would change the protein adsorption. The sample (6 mm in diameter) was immersed in phosphate-buffered saline (PBS) for 2 hours. The samples then were immersed in a bovine serum albumin (BSA (4.5 g/L); Sigma-Aldrich, St Louis, MO, USA) solution at 37°C for 12 hours. The disks then rinsed with fresh PBS, immersed in 1% sodium dodecyl sulfate (SDS)/PBS solution, and sonicated at room temperature for 20 minutes to completely detach the BSA from the scaffold.38 (link) A BCA Protein Assay Kit (Beijing Leagene Biotechnology Co., Ltd, China) was used to determine the amount of BSA adsorbed onto the sample (n=9).
Bovine serum albumin (bsa)
Manufactured by Leagene
Sourced in China
BSA is a laboratory equipment used for the measurement and analysis of protein concentration. It functions as a standard reference material for the Bradford assay, a widely used method for quantifying protein levels in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using bovine serum albumin (bsa)
1
Protein Adsorption on Nano-Coated Films
2
Immunohistochemical Analysis of Cartilage
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The sections for cartilage pellets were dewaxed and rehydrated and then incubated with 3% H2O2. After antigen retrieval, blocked with 5% bovine serum albumin (BSA, Leagene, China). The sections were incubated with primary antibody (Collagen II, 1 : 100, Cell Signaling Technology, USA; SOX9, 1 : 50, Santa Cruz Biotechnology, USA) overnight at 4°C. After washed with phosphate-buffered saline (PBS; Gibco, USA), the slides were incubated in secondary antibody (Boster, China), streptavidin-biotin complex, and 3,3′-diaminobenzidine (DAB; Boster, China) follow one another. The stained sections onto microscope slides were evaluated by light microscopy (Leica, Germany).
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