Oligo array-CGH analysis was performed using an Agilent SurePrint G3 Human CGH 60 K microarray (AMADID 021924 Agilent Technologies, Santa Clara, CA, USA) spanning the entire human genome at a median resolution of 41 kb. Totally, 500 ng of genomic DNA from the 20q-KO and WT cells were differentially labelled by random priming with Cy5-dCTP and Cy3-dCTP. The hybridization was carried out according to the manufacturer's protocol. Arrays were scanned using an Agilent DNA Microarray scanner G2565CA (Agilent Technologies). Microarray data were extracted and visualized using Feature Extraction software v10.7 and Agilent Genomic Workbench (AGW) software v7.0 (Agilent Technologies). Copy number altered regions were detected using the Aberration Detection Method 2 (ADM-2) algorithm set as 6, with a minimum number of three consecutive probes.
Sureprint g3 human cgh 60 k microarray
Manufactured by Agilent Technologies
Sourced in United States
The SurePrint G3 Human CGH 60 K microarray is a laboratory equipment product designed for the analysis of human chromosomal variations. The microarray provides a comprehensive and high-resolution platform for the detection and identification of copy number variations across the human genome.
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Lab products found in correlation
2 protocols using sureprint g3 human cgh 60 k microarray
1
Genome-wide Copy Number Analysis
2
Genome-wide Oligo aCGH Analysis
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Oligo aCGH analysis was performed using an SurePrint G3 Human CGH 60K microarray (AMADID 021924, Agilent Technologies) spanning the entire human genome at a median resolution of 41 kb. In total, 500 ng of genomic DNA were differentially labeled by random priming with Cy5-dCTP and Cy3-dCTP. Arrays were scanned using a G2565CA scanner (Agilent Technologies). Data were extracted and visualized using Feature Extraction software, v10.7 and Genomic Workbench software, v7.0 (Agilent Technologies). Copy number altered regions were detected using the Aberration Detection Method 2 algorithm set as 6, with a minimum number of three consecutive probes.
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