The tissue sections were examined with an A1 Resonance Plus inverted microscope (Nikon, Melville, NY) equipped with a four-laser Gallium-Arsenide-Phosphide/normal Photomultiplier Tube detector unit (DU4) (GaAsP: 488 and 561; PMT: 405 and 640), Galvano resonant scanner and NIS Elements Advanced Research software (version 4.50.00). Images were acquired by sequential scanning to avoid fluorescence cross-over using a 405/488/561/640 dichroic mirror. All slides were imaged using the following bandpass filters: 405 solid-state diode laser and 450/50nm bandpass filter, 488nm solid-state diode laser and 525/50 bandpass filter, 561nm solid-state diode laser and 600/50 bandpass filter, and 640 solid-state diode laser and 685/70nm bandpass filter. Images were captured using a 60x Plan Apo lambda objective (1024 x 1024 pixels), numerical aperture 0.75, pinhole 1.2 AU, and exposure 6.2 seconds per pixel dwell. Detector sensitivity (gain) and laser power settings were kept the same for all collected images to allow comparisons between markers and cows. A total of 10 images were collected per cow to perform statistical analysis.
A1 resonance plus inverted microscope
Manufactured by Nikon
Sourced in United States
The A1 Resonance Plus inverted microscope is a high-performance laboratory instrument designed for advanced imaging and analysis. It features a sturdy inverted design, providing a stable platform for various sample types. The microscope is equipped with a resonance-based scanning system, enabling fast and precise image acquisition. It is intended for use in a range of research and scientific applications, but a detailed description of its core function is not available without potential bias or extrapolation.
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Lab products found in correlation
2 protocols using a1 resonance plus inverted microscope
1
Multicolor Imaging of Bovine Tissue
2
Fluorescence Microscopy Imaging Protocol
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The tissue sections were examined with an A1 Resonance Plus inverted microscope (Nikon, Melville, NY, USA) equipped with a four-laser Gallium-Arsenide-Phosphide/normal Photomultiplier Tube detector unit (DU4) (GaAsP: 488 and 561; PMT: 405 and 640), Galvano resonant scanner and NIS Elements Advanced Research software (version 4.50.00). Images were acquired by sequential scanning to avoid fluorescence cross-over using a 405/488/561/640 dichroic mirror. All slides were imaged using the following bandpass filters: 405 solid-state diode laser and 450/50 nm bandpass filter, 488 nm solid-state diode laser and 525/50 bandpass filter, 561 nm solid-state diode laser and 600/50 bandpass filter, and 640 solid-state diode laser and 685/70 nm bandpass filter. Images were captured using a 60× Plan Apo lambda objective (1024 × 1024 pixels), numerical aperture 0.75, pinhole 1.2 AU, and exposure 6.2 s per pixel dwell. Detector sensitivity (gain) and laser power settings were kept the same for all collected images to allow comparisons between markers and cows. A total of 10 images were collected per cow to perform statistical analysis.
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