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The RA-6A2 is a laboratory instrument designed for automated liquid handling and sample preparation. It features a robotic arm, liquid handling tips, and integrated control software to facilitate efficient and precise liquid transfer and sample processing tasks.

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Lab products found in correlation

Ctl immunospot reader, by Cellular Technology (1 mentions) Multiscreen ip plate, by Merck Group (1 mentions) Hrp conjugated streptavidin, by Southern Biotech (1 mentions) Bcip nbt, by Merck Group (1 mentions) Poly 1 c, by Merck Group (1 mentions)

2 protocols using ra 6a2

1

IFN-γ ELISPOT Assay for Mouse T Cells

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B6 and B6.E+ splenic CD4+ T cells were obtained by incubating cells with anti-CD4 microbeads for 30 min. at 4°C and flowing samples through magnetized LS positive selection columns (Miltenyi Biotec). 1.5 × 106 T cells were cultured for 48 h with equal numbers of irradiated Balb/c or SJL splenocytes, 20ng/mL phorbol 12-myristate 13-acetate (PMA) + 1μg/mL ionomycin, or medium only in 96-well MultiScreen-IP plates (Millipore) coated with purified anti-IFNγ (RA-6A2; eBioscience). Plates were then incubated with biotinylated anti-IFNγ (XMG1.2; eBioscience) and streptavidin-conjugated HRP (Southern Biotech), developed with 5-bromo-4-chloro-3-indoyl phosphate/nitro blue tetrazolium (BCIP/NBT; Sigma-Aldrich), read on a CTL ImmunoSpot reader, and analyzed using CTL ImmunoSpot 4.0 (Cellular Technology Limited).
Ni P.P., Wang Y, & Allen P.M. (2014). Both Positive and Negative Effects on Immune Responses by Expression of a Second Class II MHC Molecule. Molecular immunology, 62(1), 199-208.
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2

Bpep-NLC Modulates BMDC-Induced T Cell Responses

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BMDCs were incubated, during the last day of differentiation, with either free Bpep (1 µg/mL), Bpep-NLC (125 µg/mL), or control NLC (125 µg/mL) and then stimulated overnight with Poly(I:C) (20 µg/mL, Sigma Aldrich). T cells from BDC12-4.1 mice were enriched as described above and cocultured with the different BMDCs groups in anti-mouse IFN-γ (R4-6A2, eBioscience) coated 96-well filtration plate (Millipore Sigma) for two days. Secondary biotinylated antibody IFN-γ (RA-6A2, eBioscience) was added on the third day, and streptavidinhorseradish peroxidase the day after. The plate was then read using a Zeiss KS EliSpot reader.
Calderón‐Colón X., Zhang Y., Tiburzi O., Wang J., Raimondi G., & Patrone J. (2022). Design and Characterization of Lipid Nanocarriers for Oral Delivery of Immunotherapeutic Peptides.
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