Quantum fx μct

Manufactured by PerkinElmer

Sourced in United States

The Quantum FX μCT is a high-resolution micro-computed tomography (micro-CT) system designed for non-destructive imaging and analysis of small samples. It provides 3D visualization and quantification of the internal structure and composition of a wide range of materials and specimens.

Automatically generated - may contain errors

Lab products found in correlation

3 matic software, by Materialise (1 mentions) Quantum gx software, by PerkinElmer (1 mentions)

4 protocols using quantum fx μct

Quantifying Cement Distribution in Bone

Check if the same lab product or an alternative is used in the 5 most similar protocols

To determine the location and distribution of injected cement, micro-computed tomography (Quantum FX μCT; Perkin Elmer Co. Ltd., USA) was performed using 148 μm thick slices. Images were saved in the Digital Imaging and Communications in Medicine format and then analyzed using MIMICS software, version 21 (Materialise Co. Ltd., Belgium). This software was developed for medical image segmentation and 3D model reconstruction. Hounsfield units were adjusted for each structure in the CT system, using 3,000–6,500 HUs for the cement. The geometry of the cement was then measured using 3-matic software (Materialise Co. Ltd.).
To determine the distribution of injected cement, we measured x-, y-, and z-axis dimensions. The direction of the hole was designated as the x-axis to examine the effect of cement distribution exiting through the fenestrated hole. The center of the injected cement was calculated using the analytical fitting function, and the distance was measured between the center and the screw tip.

Sung S., Kwon J.W., Park T.H., Lee S.B., Moon S.H, & Lee B.H. (2022). Biomechanical Comparison and Three-Dimensional Analysis of Cement Distribution Patterns for Different Pedicle Screw Designs. BioMed Research International, 2022, 8293524.

+ Open protocol

+ Expand

Cone-Beam μCT Imaging of Rat Knee Joints

Check if the same lab product or an alternative is used in the 5 most similar protocols

A type of bench-top cone-beam animal scanner (Quantum FX μCT, Perkin Elmer, USA) was used to analyze the hind knee joints of each rat. All specimens were scanned with the following parameters: 90 kV/160 μA, pixel size of 148 μm, and a 0.5 mm aluminum filter. The ABT263-treated knees and the contralateral controls were scanned separately. After scanning, a total of 512 cross-sectional slices were generated and collected, and then three-dimensional (3D) reconstruction of each sample was accomplished.

Yang H., Chen C., Chen H., Duan X., Li J., Zhou Y., Zeng W, & Yang L. (2020). Navitoclax (ABT263) reduces inflammation and promotes chondrogenic phenotype by clearing senescent osteoarthritic chondrocytes in osteoarthritis. Aging (Albany NY), 12(13), 12750-12770.

+ Open protocol

+ Expand

Orthotopic Injection of Lung Cancer Cells in Mice

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

S/Trp53^—/—, and S/P/E/Trp53^—/— LBSCs were injected orthotopically into the left lungs (final volume 50 ml) of 8~10 weeks old wild-type C57BL/6 mice (both male and female) using a 30-gauge needle. For IVIS and μCT imaging, mice were anaesthetized by isoflurane inhalation, administered 150 mg kg⁻¹ d-luciferin by intraperitoneal injection and placed in a mouse imaging shuttle device that maintains the sedated mouse in a stationary position. At 8 minutes after luciferin injection, bioluminescence was measured using a Perkin Elmer IVIS imaging system (Caliper Life Sciences-Xenogen, Hopkinton, MA). For μCT imaging, mice were scanned for 4 min under isoflurane anesthesia using a small animal Quantum FX μCT (PerkinElmer) at 45 μm resolution, 90 kV, with 160 μA current. Images were acquired using PerkinElmer Quantum GX software. The bioluminescence optical and CT datasets were co-registered in 3-D using the Living Image 4.5 software. Mouse lung tissues were prepared for histology and hematoxylin/eosin staining was per- formed as previously described (Regala et al., 2009 (link)). All animal experiments were performed under an approved IACUC protocol of Mayo Clinic.

Liu Y., Yin N., Wang X., Khoor A., Sambandam V., Ghosh A.B., Fields Z.A., Murray N.R., Justilien V, & Fields A.P. (2020). Chromosome 3q26 Gain Is an Early Event Driving Coordinated Overexpression of the PRKCI, SOX2, and ECT2 Oncogenes in Lung Squamous Cell Carcinoma. Cell reports, 30(3), 771-782.e6.

+ Open protocol

+ Expand

Microstructure Analysis of Proximal Tibia

Check if the same lab product or an alternative is used in the 5 most similar protocols

The right proximal tibiae were scanned with Quantum FX μ-CT (Perkin Elmer) at an energy level of 90 kV and intensity of 0.16 mA with a field of view of 10 mm (voxel size, 20 μm; scanning time, 3 min). Scanning for the proximal tibia was initiated from the level of knee joint cavity to tibia distally for 512 slices totally. Evaluations were performed on 100 slices beginning from the slice which was defined as the plane where the growth plate had just disappeared at the cross-section. Bone morphometric measurements, including Bone Mineral Density (BMD, mg/cc), Bone Volume/Total Volume (Bv/Tv), trabecular separation (Tb.Sp, mm) and Trabecular Pattern Factor (Tb.Pf, mm⁻¹) were calculated by the software Inveon research workplace. The value of bone morphometric parameters were shown as mean ± SD. Student’s t-test was used to evaluate statistically significant differences between the values. A p value less than 0.05 was considered statistically significant and correlations were considered high significance at the p value less than 0.01 level.

Liu C., Zhao Y., He W., Wang W., Chen Y., Zhang S., Ma Y., Gohda J., Ishida T., Walter T.S., Owens R.J., Stuart D.I., Ren J, & Gao B. (2015). A RANKL mutant used as an inter-species vaccine for efficient immunotherapy of osteoporosis. Scientific Reports, 5, 14150.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!