Fasting blood samples of approximately 5 ml for measuring complete blood count (Sysmex K-1000, Bohemia, NY, USA) and other factors were immediately centrifuged at 3000 g for 10 min. Serum levels of blood urea nitrogen (BUN), creatinine (Cre), fasting glucose, total cholesterol (TCH), triglyceride (TG), high-density lipoprotein cholesterol (HDL-cholesterol), and low-density lipoprotein cholesterol (LDL-cholesterol) were measured using an autoanalyzer (COBAS Integra 800, Roche Diagnostics, Basel, Switzerland). Blood samples were assayed for NT-proBNP by electrochemiluminescence immunoassay on the Elecsys 2010 Immunoanalyzer (Roche Diagnostics, Indianapolis, IN, USA).
Elecsys 2010 immunoanalyzer
Manufactured by Roche
Sourced in United States, China
The Elecsys 2010 Immunoanalyzer is a fully automated, random-access immunoassay analyzer used for the determination of a wide range of analytes in clinical laboratories. It is designed to provide accurate and reproducible results with high throughput.
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5 protocols using elecsys 2010 immunoanalyzer
1
Comprehensive Metabolic Profile Analysis
2
Serum Biochemical Analyses in Research
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Fasting blood samples (approximately 5 mL) were immediately centrifuged at 3000 g for 10 minutes after collection. Serum samples were stored at 4°C and used for biochemical analyses within 1 h of collection. Serum levels of creatinine fasting glucose, total cholesterol, triglycerides, high-density lipoprotein-cholesterol, albumin, globulin, total calcium, and phosphorus were measured using an autoanalyzer (COBAS Integra 800, Roche Diagnostics, Basel, Switzerland). Serum samples were assayed for NT-pro-BNP by an electrochemiluminescence immunoassay on the Elecsys 2010 Immunoanalyzer (Roche Diagnostics, Indianapolis, IN, USA) [15 (link)16 (link)]. Serum intact parathyroid hormone (iPTH; Diagnostic Systems Laboratories, Webster, Texas, USA) was measured using a commercially available enzyme-linked immunosorbent assay [14 (link)].
3
Biomarkers during Cardiac Surgery
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In this study, 5 mL of arterial blood was collected into tubes containing sodium citrate at four different time points: T1, after anesthesia induction before administration of MP; T2, 30 minutes after initiation of CPB; T3, 5 minutes after administration of protamine; and T4, 6 hours after cessation of CPB. Plasma was separated immediately by centrifugation and was stored at À70 C until analysis. Interleukin-6 (IL-6), IL-8, and IL-10 were determined at time points T1 to T4 by using commercial enzyme-linked immunosorbent assay kits (Quantikine, R&D Systems, Abington, United Kingdom). Total plasma concentration of MP was determined at time points T2 to T4 by using a high-performance liquid chromatographyelectrospray-tandem mass spectrometry method [17] . The lower limit of MP quantification was 2 ng/mL, and the interday coefficient of variation was less than 10%. Troponin T concentrations were determined using an electrochemiluminescence immunoassay (Elecsys 2010 immunoanalyzer, Roche Diagnostics, Berlin, Germany) at time points T1, T3, and T4 and at 6 o'clock on the first postoperative morning. Blood glucose was collected for study purposes at time points T1, T2, T3, and T4 and at 6 o'clock on the first postoperative morning.
4
Quantitative Hepatitis B Virus Monitoring
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Serum HBV DNA levels were quantified using Roche COBAS TaqMan HBV Test (F. Hoffmann-La Roche Ltd., Basel Switzerland), with the lower detection limit threshold set at 20 IU/ml. Serum HBsAg levels were quantified using Roche Elecsys HBsAg II quant assay (Roche Diagnostics, China; the lower detection limit threshold was 0.05 IU/mL). The quantification of serum HBeAg and anti-HBe levels were performed based on the cut-off index (COI) by Roche Elecsys 2010 immunoanalyzer (Roche Diagnostics, China). ALT was measured locally in accordance with standard procedures. HBV genotype was performed using genotype-specific primers33 (link).
Response to treatment was defined as a composite endpoint of HBeAg loss with HBV DNA level <2,000 IU/mL (~10,000 copies/mL) and ALT normalization at the end of 72 weeks (48 weeks of treatment and 24 weeks of follow-up)30 (link).
Response to treatment was defined as a composite endpoint of HBeAg loss with HBV DNA level <2,000 IU/mL (~10,000 copies/mL) and ALT normalization at the end of 72 weeks (48 weeks of treatment and 24 weeks of follow-up)30 (link).
5
Quantitative Biomarker Monitoring in Chronic Hepatitis B
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The serum samples for clinical indicator measurement were obtained from fresh whole blood collected in tube without anticoagulants. All of the clinical indicators were quantified in samples taken at baseline and during treatment period and follow-up periods. Sampling was performed at weeks 0, 4, 8, 12, 24, 36, 48, and 72 (week 0 was the time-point at which treatment began). HBV DNA quantification was measured using the Roche COBAS TaqMan HBV Test (F. Hoffman-La Roche Ltd., Basel, Switzerland) with a lower limit of detection <20 IU/ml. HBsAg was quantifed using the Roche Elecsys HBsAg II quant assay (Roche Diagnostics, China). HBeAg was measured using a Roche Elecsys 2010 Immunoanalyzer (Roche Diagnostics, China). Serum ALT was detected at the time of sampling in accordance with standard procedures. HBV genotyping performed using polymerase chain reaction (PCR) assays and genotype-specific primers (20 (link)).
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