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Glucose oxidase ec 1

Manufactured by Merck Group
Sourced in United States

Glucose oxidase (EC 1.1.3.4) is an enzyme that catalyzes the oxidation of glucose to gluconic acid and hydrogen peroxide. It is commonly used in the food and pharmaceutical industries for the determination of glucose concentration.

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2 protocols using glucose oxidase ec 1

1

Glucose Oxidase Activity Assay

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glucose oxidase (EC 1.1.3.4, type X-S from Aspergillus niger, 117200 U/G solid), glucose, methyl red sodium salt and dextrose anhydrous were purchased from Sigma-Aldrich (St. Louis, MO, USA). Deionized ultra-filtered water (Ω > 18 MΩ.cm, Fisher scientific, Pittsburgh, PA, USA).
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2

Electrochemical Biosensor for Glucose Detection

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Reagents used: sodium chloride, dipotassium hydrogen phosphate trihydrate, sodium hydroxide, glucose, and acetic acid (Diakon, Russia); iron(III) chloride, potassium chloride, calcium chloride, and sodium carbonate (Khimmed, Russia); hydrochloric acid, hydrogen peroxide (30% solution), sodium carbonate, and potassium hexacyanoferrate(III) (HCF) (Reakhim, Russia); chitosan (low molecular weight), sodium polystyrene sulfonate (PSS, 70 kDa), poly(allylamine hydrochloride) (PAH, 70 kDa), ethylenediaminetetraacetic acid (EDTA), and 4-aminoantipyrine, phenol, glucose oxidase (EC 1.1.3.4) from Aspergillus niger (activity, 185000 U/g) (Sigma-Aldrich, USA). Taunit-M multiwalled carbon nanotubes (NanoTechCentre LLC, Tambov, Russia) were used for modification of the electrode.
Screen-printed three-contact electrodes (Color Electronics, Moscow, Russia) containing Electrodag 6017SS graphite paste (Henkel, Germany) were used.
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