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Meso sector s 600

Manufactured by Mesoscale
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The MESO SECTOR S 600 is a laboratory instrument designed for the sectioning of materials. It is a precision tool used to prepare samples for further analysis or examination. The MESO SECTOR S 600 is capable of producing thin, uniform sections of materials with a high degree of accuracy and repeatability.

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Lab products found in correlation

Plate shaker, by Thermo Fisher Scientific (2 mentions) Facsymphony, by BD (1 mentions) Dtx 880 multimode detector, by Beckman Coulter (1 mentions) U plex human α synuclein kit, by Mesoscale (1 mentions) Msd discovery workbench 4, by Mesoscale (1 mentions) V plex custom human biomarkers kit, by Mesoscale (1 mentions) Tumor necrosis factor (tnf), by Mesoscale (1 mentions) Tnf α, by Thermo Fisher Scientific (1 mentions) Il 1β, by Thermo Fisher Scientific (1 mentions) Rb1007, by Randox (1 mentions) Abx pentra 400, by Horiba (1 mentions)

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Meso Sector S 600 plate reader MESO Sector S 600 Instrument Sector 600

13 protocols using meso sector s 600

1

Cytokine Quantification from Cell Supernatants

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50 μl of apical and 50 μl basolateral cell culture supernatants were analyzed for cytokines using the Meso Scale Discovery (MSD) V-plex Viral Panel 1 Human Kit (Meso Scale Diagnostics, Rockville, MA) as per the manufacturer’s instructions. Briefly, 1:5 dilutions of cell supernatant samples were diluted in PBS containing 1% Triton-X. Samples were added to the MSD plate along with a 7-point 4-fold serial dilution (concentrations related to certificate of analysis for each individual standard) of protein standards diluted in PBS with 1% Triton-X. The MSD plate was sealed, and samples incubated at room temperature for 2 hours on a plate shaker (ThermoFisher Scientific, Waltham, MA) at 700RPM. The plate was washed 3x in wash buffer and 25 μl of secondary antibody was added to each well. Plates were sealed and incubated at room temperature on a plate shaker at 700RPM for a further 2 hours in the dark. Plates were washed 3x with wash buffer and 50 μl of 2x read buffer (MSD R92TC) was added to each well. The plates were read on the MESO Sector S 600 (Meso Scale Diagnostics) and concentrations determined against the standard curves.
Calvert B., Quiroz E., Lorenzana Z., Doan N., Kim S., Senger C., Wallace W., Salomon M., Henley J, & Ryan A. (2022). Neutrophilic inflammation promotes SARS-CoV-2 infectivity and augments the inflammatory responses in airway epithelial cells. bioRxiv.
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2

Quantification of Plasma Biomarkers

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Plasma insulin, TNF-α and IL-6 were quantified by electrochemiluminescence (MESO SECTOR S 600) using kits from MesoScale Diagnostics (MSD, Rockville, MD, USA).
AlAsfoor S., Rohm T.V., Bosch A.J., Dervos T., Calabrese D., Matter M.S., Weber A, & Cavelti-Weder C. (2018). Imatinib reduces non-alcoholic fatty liver disease in obese mice by targeting inflammatory and lipogenic pathways in macrophages and liver. Scientific Reports, 8, 15331.
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3

Plasma Biomarker Analysis in Sh2b3 Mice

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Plasma samples from Sh2b3E372K mice were plated for the mouse U-plex Biomarker Group 1 Multiplex Assay Box 2 10-Assay Plate (Meso Scale Discovery, cat #K15322K) and the assay performed according to the manufacturer’s instructions. The plate was read on a Meso Sector S 600 (Meso Scale Discovery).
Zhang Y., Morris R., Brown G.J., Lorenzo A.M., Meng X., Kershaw N.J., Kiridena P., Burgio G., Gross S., Cappello J.Y., Shen Q., Wang H., Turnbull C., Lea-Henry T., Stanley M., Yu Z., Ballard F.D., Chuah A., Lee J.C., Hatch A.M., Enders A., Masters S.L., Headley A.P., Trnka P., Mallon D., Fletcher J.T., Walters G.D., Šestan M., Jelušić M., Cook M.C., Athanasopoulos V., Fulcher D.A., Babon J.J., Vinuesa C.G, & Ellyard J.I. (2024). Rare SH2B3 coding variants in lupus patients impair B cell tolerance and predispose to autoimmunity. The Journal of Experimental Medicine, 221(4), e20221080.
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4

Tumor Cell Killing by BiTE-Activated T Cells

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Dissociated tumor cells (target cells) were co-cultured with human T cells (effector cells) at an effector-to-target (E:T) cell ratio of 10:1 in the presence of HLE BiTE® molecules. After a 48h incubation, cells were analyzed for T cell-dependent cellular cytotoxicity (TDCC), T-cell activation and cytokine production. Tumor cell viability was assessed by flow cytometry using an antibody to human EpCAM (Biolegend) or TO-PRO-3 Iodide (Thermo Fisher) to identify dead cells. T-cell activation was assessed by flow cytometry using antibodies to CD25 and CD69 (BD Biosciences). Cells were incubated with antibodies for 30 minutes at 4°C, washed in FACS buffer and analyzed on a FACSymphony™ (BD Biosciences). Cytokines in the supernatants were quantified using the Human ProInflammatory Culture Kit (K15009B) on MESO Sector S600 (Meso Scale Discovery) according to the manufacturer’s instructions.
Chou J., Egusa E.A., Wang S., Badura M.L., Lee F., Bidkar A.P., Zhu J., Shenoy T., Trepka K., Robinson T.M., Steri V., Huang J., Wang Y., Small E.J., Chan E., Stohr B.A., Ashworth A., Delafontaine B., Rottey S., Cooke K.S., Sadraei N.H., Yu B., Salvati M., Bailis J.M., Feng F.Y., Flavell R.R, & Aggarwal R. (2023). Immunotherapeutic targeting and PET imaging of DLL3 in small cell neuroendocrine prostate cancer. Cancer research, 83(2), 301-315.
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5

Multiplex Cytokine Analysis in Mice

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An electrochemiluminescence assay (V-PLEX Proinflammatory Panel 1 Mouse Kit K15048D-1, Meso Scale Diagnostics, LLC, Gaithersburg, MD, USA) was used for detection of the following cytokines according to the manufacturer’s instructions: IFNγ, TNFα, interleukin 1β (IL-1β), IL-2, IL-4, interleukin-5 (IL-5), interleukin-6 (IL-6), interleukin-10 (IL-10), interleukin-12p70 (IL-12p70), keratinocyte chemoattractant growth-regulated oncogene (KC/GRO). Briefly, antibody solutions were added at room temperature, incubated and washed as per manufacturers protocol. The 96-well plate was immediately analyzed using a plate reader (Meso Sector S 600, Meso Scale Diagnostics).
Mauda-Havakuk M., Hawken N.M., Owen J.W., Mikhail A.S., Saxena A., Karim B., Wakim P.G., Pritchard W.F., Karanian J.W, & Wood B.J. (2022). Comparative analysis of the immune response to RFA and cryoablation in a colon cancer mouse model. Scientific Reports, 12, 18229.
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6

Serum Cytokine Profiling by Protein Array

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The systemic concentration of Th1 interleukin-6 (IL-6), and Th2 interleukin-13 (IL-13) in serum specimens collected from POD-7 animals were analyzed using commercial protein arrays (Meso Scale Diagnostics, Rockville, Maryland, USA) according to the manufacturer’s instructions. Data acquisition was performed using a Meso Sector S600 (Meso Scale Diagnostics, Rockville, Maryland, USA) and quantitative results were generated using Methodical Mind software (version MMPR 1.0.27; Meso Scale Diagnostics, Rockville, Maryland, USA).
Rowe C.J., Nwaolu U., Salinas D., Hong J., Nunez J., Lansford J.L., McCarthy C.F., Potter B.K., Levi B.H, & Davis T.A. (2023). Inhibition of focal adhesion kinase 2 results in a macrophage polarization shift to M2 which attenuates local and systemic inflammation and reduces heterotopic ossification after polysystem extremity trauma. Frontiers in Immunology, 14, 1280884.
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7

Quantification of IFN-α Levels

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IFN-α was measured using the Human IFN-α (pan-specific) ELISA kit (MabTech) and analyzed on an ELISA reader DTX 880 Multimode detector (Beckman Coulter). For LAIV experiments, the IFN-α2a Ultra-Sensitive Kit was used and data analyzed by MESO SECTOR S 600 (Meso Scale Discovery).
Bajwa G., DeBerardinis R.J., Shao B., Hall B., Farrar J.D, & Gill M.A. (2016). Critical Role of Glycolysis in Human Plasmacytoid Dendritic Cell Antiviral Responses. Journal of immunology (Baltimore, Md. : 1950), 196(5), 2004-2009.
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8

MSD Multiplex Cytokine Quantification

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50 μl of cell culture supernatants were analyzed for cytokines using the Meso Scale Discovery (MSD) Proinflammatory panel 1, Human kit, Lot:K0081459 & K0081080 (Meso Scale Diagnostics, Rockville, MD) as per the manufacturer’s instructions. Briefly, 1:5 dilutions of cell supernatant samples were diluted in PBS containing 1% Triton-X. Samples were added to the MSD plate along with a 7-point 4-fold serial dilution (concentrations related to certificate of analysis for each individual standard) of protein standards diluted in PBS with 1% Triton-X. The MSD plate was sealed, and samples incubated at room temperature for 2 hours on a plate shaker (ThermoFisher Scientific) at 700RPM. The plate was washed 3x in wash buffer and 25 μl of secondary antibody was added to each well. Plates were sealed and incubated at room temperature on a plate shaker at 700RPM for a further 2 hours in the dark. Plates were washed 3x with wash buffer and 50 μl of 2x read buffer (MSD R92TC) was added to each well. The plates were read on the MESO Sector S 600 (Meso Scale Diagnostics), and concentrations determined against the standard curves.
Calvert B.A., Quiroz E.J., Lorenzana Z., Doan N., Kim S., Senger C.N., Anders J.J., Wallace W.D., Salomon M.P., Henley J, & Ryan A.L. (2023). Neutrophilic inflammation promotes SARS-CoV-2 infectivity and augments the inflammatory responses in airway epithelial cells. Frontiers in Immunology, 14, 1112870.
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9

Aβ Peptide Levels in rTg9191 Mice

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Total Aβ38, Aβ40, and Aβ42 protein levels in the water-soluble (TBS), detergent-soluble (TBS-TX) and insoluble (FA) fractions of brain extracts of rTg9191 mice at young (4-month-old, n = 3 (1 male and 2 females)), middle (12-month-old, n = 5 (2 males and 3 females)), and old (21-month-old, n = 3 (1 male and 2 females); and 24-month-old, n = 5 (2 males and 3 females)) ages were measured using a multi-plex ELISA for Aβ38, Aβ40, and Aβ42 (N45148A-1, Meso Scale Discovery, Rockville, MD) following the manufacturer’s instructions. In this assay, C-terminal end-specific antibodies against Aβ38, Aβ40, and Aβ42 are used as immunocapture reagents, and mouse monoclonal antibody 6E10 directed against amino acids 1–16 of human Aβ is used as the detection antibody. Signals were detected and quantified using a Meso Sector S 600 plate reader (MesoScale Discovery). Samples were analyzed in duplicate, and the mean values are reported.
Liu P., Paulson J.B., Forster C.L., Shapiro S.L., Ashe K.H, & Zahs K.R. (2015). Characterization of a Novel Mouse Model of Alzheimer’s Disease—Amyloid Pathology and Unique β-Amyloid Oligomer Profile. PLoS ONE, 10(5), e0126317.
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10

Quantification of α-synuclein Levels

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Preparation of cell lysates and quantification of total protein concentration were performed using the same procedure as for western blot analysis. α-synuclein protein concentrations were measured with U-PLEX Human α-synuclein Kit (Meso Scale Discovery, Rockville, MD) and MESO SECTOR S 600 (Meso Scale Discovery) as per the manufacturer’s instructions. The biotinylated capture antibody and SULFO-TAG-conjugated detection antibody that we used in the assay were those packaged in the kit. To compare among conditions, quantified α-synuclein concentrations were normalized by total protein concentrations.
Suzuki H., Egawa N., Imamura K., Kondo T., Enami T., Tsukita K., Suga M., Yada Y., Shibukawa R., Takahashi R, & Inoue H. (2024). Mutant α-synuclein causes death of human cortical neurons via ERK1/2 and JNK activation. Molecular Brain, 17, 14.
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