For histologic analysis, we chose the associated humerus. Before sectioning, photography, photogrammetry, and CT scanning of the humerus were conducted. Molding and casting were made from the specimen for later research. The mid-shaft of the humerus was sampled for the examination. The sample was prepared and transversely thin sectioned following Lamm (2013) . The slides were examined using Nikon Eclipse E600 POL petrographic polarizing microscope with a lambda 530 nm plate. The bone cross-section was photographed using a combination of Nikon DS-Ri2 camera and NIS-Elements BR (ver. 4.13) software. Adobe Photoshop (ver. 21.2) is used for image enhancement and tracing LAGs. Bone wall thickness and area of the transverse section were quantified by Image J (ver. 1.53; Schneider, Rasband & Eliceiri, 2012 (link)).
Ds ri2 camera
Manufactured by National Instruments
Sourced in Japan
The DS-Ri2 is a digital camera designed for microscopy applications. It features a high-resolution CMOS sensor and supports live image capture, image acquisition, and image storage capabilities. The core function of the DS-Ri2 is to enable the capture and documentation of microscopic samples and specimens.
Automatically generated - may contain errors
Lab products found in correlation
Hema 3 staining kit, by Thermo Fisher Scientific (3 mentions)
Shandon cytospin 3, by Thermo Fisher Scientific (2 mentions)
Nis elements br, by National Instruments (1 mentions)
Eclipse e600, by Nikon (1 mentions)
Eclipse ti2 microscope, by Nikon (1 mentions)
Ab3523, by Abcam (1 mentions)
Eclipse ni e, by Nikon (1 mentions)
Dabco, by Merck Group (1 mentions)
Ti 2000 fluorescence microscope, by Nikon (1 mentions)
Provis ax70 light microscope, by Nikon (1 mentions)
Histocore arcadia, by Leica (1 mentions)
Binocular microscope, by Olympus (1 mentions)
Smz1270 stereo microscope, by Nikon (1 mentions)
8 protocols using ds ri2 camera
1
Histologic Analysis of Humerus Samples
2
Morphological Characterization of FTM Particles
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The gross morphology of dry FTM particles was observed under a light microscope (Nikon Eclipse Ti2 microscope). FTM behaviour in the dissolution medium was monitored by placing 20–25 FTM particles with 3 drops (~150 µL) of dissolution medium in a well (96-well microplate). The plate was covered to minimise solvent evaporation, and images of the particles observed under the microscope at 2, 15, 30 and 60 min were taken (Nikon DS-Ri2 camera) and processed (NIS-Elements BR Analysis 4.60.00).
3
Immunofluorescence assay for NF-κB and iNOS
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Cells were seeded on cover glasses and incubated with different supplemented media according to experimental groups. After treatment, cells were washed with ice cold PBS buffer and fixed with cold 4% formaldehyde solution in PBS for 10 minutes. Fixed cells were incubated with the primary antibodies rabbit anti-NFκB (1/100, 82425, Cell Signaling) and rabbit anti-iNOS (1/100, ab3523, Abcam) overnight at 4°C. Primary antibodies were removed, and cells were washed with PBS and incubated with the secondary antibody anti-rabbit-FITC (1/50, sc-2012, Santa Cruz Biotechnology) at room temperature for 2 hours. After incubation with secondary antibodies, cells were washed with PBS and nuclei were stained with DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) before mounting with glycerol with Dabco® (Sigma-Aldrich) antifading. Images were taken with Nikon Eclipse Ni-E, DS-Ri2 camera, total magnification 400X (10X ocular lenses and 40X objective), NIS Elements camera software.
4
Cytospins for Hematopoietic Cell Morphology
5
Hematopoietic Differentiation Cell Cytology
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Approximately 200,000 cells from liquid hematopoietic differentiation cultures were washed twice with PBS containing 2% FBS and resuspended in PBS. Cytospins were prepared on slides using a Shandon CytoSpin III cytocentrifuge (Thermo Electron Corporation). Slides were then air-dried for 30 minutes and stained with the Hema 3 staining kit (Fisher Scientific Company LLC). The slides were read on a Nikon Eclipse Ci microscope and digital images were taken with a Nikon DS-Ri2 camera and NIS-Elements D4.40.00 software.
6
Hematopoietic Differentiation Cell Analysis
7
Histopathological and Apoptotic Analysis of Rat Intestinal Tissue
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Intestinal tissue samples obtained at the point 5 cm from the pylorus were assayed. For histopathological evaluation with a light microscope, intestinal tissues were freshly excised and fixed in 10% neutralized formalin. The tissues were then processed by routine tissue techniques using a Tissue-Tek® VIP™ 5 Jr Tissue Processor (SAKURA, Staufen, Germany) and embedded in paraffin using HistoCore Arcadia (Leica, Wetzlar, Germany). Paraffin-embedded specimens were cut into 4-μm-thick sections. The sections were mounted on slides and stained with hematoxylin and eosin (H&E). The slides were examined to detect any morphological changes in the tissue using an Olympus PROVIS AX70 light microscope (Tokyo, Japan), Nikon DS-Ri2 camera (Tokyo, Japan), and NIS-Elements BR 4.50.00 software (Tokyo, Japan).
Besides, to detect apoptosis, TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling) staining was conducted on the rat intestinal tissues and the cells according to the manufacturer's instructions (Hoffmann-La Roche, Basel, Switzerland). All fluorescent images were analyzed with the Ti-2000 fluorescence microscope (Nikon, Minato-ku, Tokyo, Japan), and the apoptotic indices were calculated based on TUNEL-positive nuclei and total nuclei.
Besides, to detect apoptosis, TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling) staining was conducted on the rat intestinal tissues and the cells according to the manufacturer's instructions (Hoffmann-La Roche, Basel, Switzerland). All fluorescent images were analyzed with the Ti-2000 fluorescence microscope (Nikon, Minato-ku, Tokyo, Japan), and the apoptotic indices were calculated based on TUNEL-positive nuclei and total nuclei.
8
Tomato Embryo Isolation and Analysis
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To isolate embryos, tomato seeds were imbibed for several hours in water, and then the seeds were dissected into embryos and endosperms by using a surgical blade and very fine tweezers under a binocular microscope (Olympus, Waltham, MA, USA). The SCBAs were performed according to Lee et al., (2010) . Briefly, isolated embryos were placed on a layer of embryo-less endosperms that laid on a MS medium. Embryos growth and greening were analyzed after 72h as described above (See material and Methods).
Histochemical detection of GUS activity was performed using 5-bromo-4chloro-3-indolyl-b-D-glucuronide as described in Donnelly et al., (1999) .
Samples were photographed under a Nikon SMZ1270 stereo microscope equipped with a Nikon DS-Ri2 camera and NIS-ELEMENT software.
Histochemical detection of GUS activity was performed using 5-bromo-4chloro-3-indolyl-b-D-glucuronide as described in Donnelly et al., (1999) .
Samples were photographed under a Nikon SMZ1270 stereo microscope equipped with a Nikon DS-Ri2 camera and NIS-ELEMENT software.
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