Cells were rinsed with ice-cold lysis buffer (50 mM, Tris, 10 mM EDTA, 1% v/v Triton-X100), supplemented with the protease inhibitor cocktail set III (80 μM aprotinin, 5 mM bestatin, 1.5 mM leupeptin, 1 mM pepstatin; Calbiochem, San Diego, CA, USA), 2 mM phenylmethylsulfonyl fluoride and 1 mM Na3VO4, then sonicated and centrifuged at 13,000× g for 10 min at 4 °C. Twenty μg of extracted proteins were subjected to SDS-PAGE and probed with the following antibodies: anti-Pgp (C219; Calbiochem), anti-MRP1 (MRPm5; Abcam, city, UK), anti-BCRP (M-70; Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA), anti-claudin 3 (PA5-16867; ThermoFisher Scientific, Waltham, MA, USA), anti-claudin 5 (4C3C2; ThermoFisher Scientific), anti-occludin (6HCLC; ThermoFisher Scientific), anti-ZO-1 (40-2200; ThermoFisher Scientific), anti-β-tubulin (D-10 and TUJ1; Santa Cruz Biotechnology Inc.), followed by a peroxidase-conjugated secondary antibody (Bio-Rad Laboratories). The membranes were washed with Tris-buffered saline-Tween 0.1% v/v solution, and the proteins were detected by enhanced chemiluminescence (Bio-Rad Laboratories).
Anti β tubulin d 10 and tuj1
Manufactured by Santa Cruz Biotechnology
Sourced in United States, Canada
Anti-β-tubulin (D-10 and TUJ1) is a lab equipment product that functions as an antibody. It is used to detect and analyze the presence of β-tubulin, a key component of the cytoskeleton in eukaryotic cells.
Automatically generated - may contain errors
2 protocols using anti β tubulin d 10 and tuj1
1
Quantification of Multidrug Resistance Proteins
2
Western Blot Analysis of Drug Transporters
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells were rinsed with ice-cold lysis buffer (50 mM, Tris, 10 mM EDTA, 1% v/v Triton-X100), supplemented with the protease inhibitor cocktail set III (80 μM aprotinin, 5 mM bestatin, 1.5 mM leupeptin, 1 mM pepstatin; Calbiochem, San Diego, CA), 2 mM phenylmethylsulfonyl fluoride (PMSF) and 1 mM Na 3 VO 4 , then sonicated and centrifuged at 13,000g for 10 min at 4 • C. 20 μg of extracted proteins were subjected to SDS-PAGE and probed with the following antibodies: anti-Pgp (C219; Calbiochem), anti-BCRP (M-70; Santa Cruz Biotechnology Inc., Santa Cruz, CA), anti-β-tubulin (D-10 and TUJ1; Santa Cruz Biotechnology Inc.), followed by a peroxidase-conjugated secondary antibody (Bio-Rad Laboratories, Hercules, CA). The membranes were washed with Trisbuffered saline-Tween 0.1% v/v solution, and the proteins were detected by enhanced chemiluminescence (Bio-Rad Laboratories).
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