Frozen liver and M. rectoris femoris samples from three animals per group were embedded in Tissue-Tek O.C.T. Compound (Sakura Finetek Europe, AJ Alphen aan den Rijn, the Netherlands) and cryosectioned in 15 µm slices at −20 °C using a CryoStar NX50 microtome (Thermo-Scientific, Germany). Liver and M. rectus femoris sections were stained with Oil Red O Stain Kit (ScyTek Laboratories Logan, UT, USA) and Haematoxylin and Eosin Fast Staining Kit (Morphisto, Offenbach, Germany), respectively, according to the manufacturer´s protocol. Stained sections were photographed with an EVOS M5000 microscope (Thermo Fisher Scientific, Dreieich, Germany).
Oil red o stain kit
Manufactured by ScyTek Laboratories
Sourced in United States
The Oil Red O stain kit is a laboratory tool used for the detection and visualization of lipids in histological samples. The kit contains the necessary components to perform the Oil Red O staining procedure, which is a common technique in cell biology and histology research.
Automatically generated - may contain errors
Lab products found in correlation
Evos m5000 microscope, by Thermo Fisher Scientific (1 mentions)
Cryostar nx50 microtome, by Thermo Fisher Scientific (1 mentions)
Tissue tek oct compound, by Sakura Finetek (1 mentions)
Dmi3000 microscope, by Leica (1 mentions)
Cellsens dimension software, by Olympus (1 mentions)
Hm340e, by Thermo Fisher Scientific (1 mentions)
Analysis ts, by Olympus (1 mentions)
Direct red 80, by Merck Group (1 mentions)
5 protocols using oil red o stain kit
1
Histological Analysis of Muscle and Liver
2
Lipid Quantification in Cardiac Tissues
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Pig LV tissue sections and rat ventricular cardiomyocytes (RV‐40 strain) were subjected to an Oil red O stain kit (ScyTek Laboratories, Inc) according to the manufacturer's directions. Sections were mounted and visualized using an Olympus DP70 microscope (for pig LV tissues) and a Leica Dmi3000 microscope (for rat ventricular cardiomyocytes). Lipid (neural fat stained by Oil red O) was quantified by analyzing the magnified (×40) images using Cellsens Dimension software (Olympus, Tokyo, Japan) and counting the number of red stain pixels, using porcine fat for the positive control.
3
Muscle Fat Analysis via Oil Red O
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Cryosections of snap‐frozen muscles were stained with Oil Red O stain kit (ScyTek, ORK‐1‐IFU) for fat analysis.
4
Neutral Lipid Quantification in Liver Cryo
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Cryosections of snap-frozen liver were stained with an Oil Red O stain kit (ScyTek, ORK-1-IFU) for neutral TG and lipid analyses.
5
Histological Liver Analysis for Fibrosis and Lipid Accumulation
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Livers were fixed in 10% phosphate-buffered formalin, routinely processed, and then embedded in paraffin. Tissue sections (6 mm) were prepared with a microtome (HM-340E; Thermo Fisher Scientific Inc., Waltham, MA). Sections were placed onto glass slides. Hematoxylin and eosin staining was performed according to standard techniques.
For Oil Red O staining, frozen liver sections were prepared as usual. Oil Red O staining was performed with the use of the Oil Red O stain kit (ScyTek Laboratories, Logan, UT) according to the manufacturer's protocols. Data were expressed as percentage of Oil Red Oepositive area per field. To observe fibrosis levels in liver tissues, liver sections were subjected to Sirius Red staining (saturated aqueous solution of picric acid containing Direct Red 80; Sigma-Aldrich, St. Louis, MO). Hepatic collagen deposition was quantified by measuring Sirius Redepositive area per total liver section (analySIS TS; Olympus Corp., Tokyo, Japan). Total liver section images were analyzed for each animal with the use of a light microscope (BX-51; Olympus Corp.) and digital imaging software [analySIS TS software version 5.1 (build 1692); Olympus Corp.].
For Oil Red O staining, frozen liver sections were prepared as usual. Oil Red O staining was performed with the use of the Oil Red O stain kit (ScyTek Laboratories, Logan, UT) according to the manufacturer's protocols. Data were expressed as percentage of Oil Red Oepositive area per field. To observe fibrosis levels in liver tissues, liver sections were subjected to Sirius Red staining (saturated aqueous solution of picric acid containing Direct Red 80; Sigma-Aldrich, St. Louis, MO). Hepatic collagen deposition was quantified by measuring Sirius Redepositive area per total liver section (analySIS TS; Olympus Corp., Tokyo, Japan). Total liver section images were analyzed for each animal with the use of a light microscope (BX-51; Olympus Corp.) and digital imaging software [analySIS TS software version 5.1 (build 1692); Olympus Corp.].
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