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Maldi tof ms materials

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The MALDI-TOF MS (Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry) materials provided by Bruker are a suite of instruments and accessories designed for high-performance mass spectrometry analysis. These materials enable the ionization and detection of a wide range of biomolecules, including proteins, peptides, and other analytes. The core function of the MALDI-TOF MS materials is to facilitate precise mass measurements and identification of the target analytes.

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Lab products found in correlation

Ubiquitin monomer, by Merck Group (3 mentions) Dithiothreitol (dtt), by Merck Group (3 mentions) Bovine serum albumin (bsa), by Merck Group (2 mentions) Hbx 41 108, by Bio-Techne (2 mentions) Pimozide, by Bio-Techne (2 mentions) Degrasyn wp1130, by Bio-Techne (2 mentions) L434078, by Merck Group (2 mentions) Pr 619, by Merck Group (2 mentions) P22077, by Merck Group (2 mentions) Gliotoxin, by Merck Group (1 mentions)

Close spelling variants of this product

MALDI-TOF MS MALDI-TOF

3 protocols using maldi tof ms materials

1

Ubiquitin Modification and Analysis

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Ubiquitin monomer, BSA, Tris, DTT and Gliotoxin were purchased from Sigma-Aldrich. MALDI TOF MS materials (targets, matrix and protein calibration mixture) were purchased from Bruker Daltonics (Bremen, Germany). PYR41 and Nutlin3A compounds were kindly provided by Sara Buhrlage (Dana-Farber Cancer Institute) and Compound-1 was kindly provided by Satpal Virdee (MRC PPU, Dundee).
De Cesare V., Johnson C., Barlow V., Hastie J., Knebel A, & Trost M. (2018). The MALDI-TOF E2/E3 Ligase Assay as Universal Tool for Drug Discovery in the Ubiquitin Pathway. Cell Chemical Biology, 25(9), 1117-1127.e4.
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2

Diubiquitin Topoisomer Characterization

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Ubiquitin monomer, BSA, Tris and DTT were purchased from Sigma-Aldrich. Diubiquitin topoisomers (M1, K6, K11, K27, K29, K33, K48 and K63-linked) were purchased from Boston Biochem (Boston, MA), additional K27 diubiquitin was produced in-house57 (link)58 , whereas all MALDI-TOF MS materials (targets, matrix and protein calibration mixture) were purchased from Bruker Daltonics (Bremen, Germany). PR-619 and P22077 (Calbiochem/Merck, Darmstadt, Germany) as well as HBX 41,108, pimozide and degrasyn/WP1130 (Tocris Bioscience, Bristol, UK) and L434078 (Sigma-Aldrich, St Louis, MO, USA) were purchased commercially. Febuxostat, SJB3-019A, compound 16, NSC 697923 and BAY 11-7082 were synthesized (Supplementary Table 3).
Ritorto M.S., Ewan R., Perez-Oliva A.B., Knebel A., Buhrlage S.J., Wightman M., Kelly S.M., Wood N.T., Virdee S., Gray N.S., Morrice N.A., Alessi D.R, & Trost M. (2014). Screening of DUB activity and specificity by MALDI-TOF mass spectrometry. Nature Communications, 5, 4763.
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3

Quantitative Analysis of Diubiquitin Linkages

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Ubiquitin monomer, Bovine Serum Albumin (BSA), Tris and DTT were purchased from Sigma Aldrich. Diubiquitin topoisomers (M1, K6, K11, K27, K29, K33, K48 and K63-linked) were purchased from Boston Biochem (Boston, MA), additional K27 diubiquitin was produced in-house57 (link), 58 (link), whereas all MALDI-TOF MS materials (targets, matrix and protein calibration mixture) were purchased from Bruker Daltonics (Bremen, Germany). PR-619 and P22077 (Calbiochem/Merck, Darmstadt, Germany) as well as HBX 41,108, Pimozide and Degrasyn/WP1130 (Tocris Bioscience, Bristol, UK) and L434078 (Sigma-Aldrich, St. Louis, MO, USA) were purchased commercially. Febuxostat, SJB3-019A, Compound 16, NSC 697923 and BAY 11-7082 were synthesized (Supplementary Table 3).
Ritorto M.S., Ewan R., Perez-Oliva A.B., Knebel A., Buhrlage S.J., Wightman M., Kelly S.M., Wood N.T., Virdee S., Gray N.S., Morrice N.A., Alessi D.R, & Trost M. (2014). Screening of DUB activity and specificity by MALDI-TOF mass spectrometry. Nature Communications, 5, 4763.
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