HepG2 cell line was purchased from American Type Culture Collection (ATCC Cell Bank, USA). Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS), EDTA, and penicillin-streptomycin were purchased from Hyclone (USA). Pancreatic enzyme EDTA digestive juice was purchased from Sigma (USA). Annexin-propidium iodide (PI) and cell cycle detection kits were purchased from Becton, Dickinson and Company (BD Company, USA). Polyvinylidene difluoride membrane was purchased from Pall Life Sciences (USA). Western blot-related chemical reagents were purchased from Beyotime Biotechnology Co., Ltd. (Shanghai, China). Enhanced chemiluminescence reagent was obtained from Amersham Biosciences (USA). Bcl-2 and Bax primary antibodies and horseradish peroxidase-tagged IgG secondary antibody were purchased from Cell Signaling (USA). Other common reagents were obtained from Sangon Biotechnology Co., Ltd. (Shanghai, China). A FACSCalibur flow cytometry instrument was purchased from Becton, Dickinson and Company. A cell incubator was purchased from Thermo Electric Company (Pennsylvania, USA). Wilfortrine was purchased from Tongwei Industrial Co., Ltd. (Shanghai, China).
Western blot related chemical reagents
Manufactured by Beyotime
Sourced in China
Western blot-related chemical reagents are a set of solutions and compounds used in the Western blotting technique. These reagents are essential for various steps in the process, such as sample preparation, gel electrophoresis, protein transfer, and detection. The core function of these reagents is to facilitate the identification and quantification of specific proteins within a complex biological sample.
Automatically generated - may contain errors
Lab products found in correlation
Ecl reagent, by Cytiva (4 mentions)
Labsystem version 1.3.1 microplate reader, by Bio-Rad (3 mentions)
Pvdf membrane, by Pall Corporation (3 mentions)
Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (2 mentions)
Hrp tagged igg secondary antibody, by Cell Signaling Technology (2 mentions)
Rna extraction kit, by Thermo Fisher Scientific (2 mentions)
Trizol reagent, by Thermo Fisher Scientific (2 mentions)
Enhanced chemiluminescence reagent, by Cytiva (1 mentions)
Bax and bcl 2 primary antibodies, by Cell Signaling Technology (1 mentions)
Facscalibur flow cytometry instrument, by BD (1 mentions)
Polyvinylidene difluoride membrane, by Pall Corporation (1 mentions)
Hepg2 cell line, by American Type Culture Collection (1 mentions)
Phosphorylated pi3k, by Cell Signaling Technology (1 mentions)
Mtt powder, by Thermo Fisher Scientific (1 mentions)
Nilotinib, by Merck Group (1 mentions)
Mmp 2, by Merck Group (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Thermo Fisher Scientific (1 mentions)
Mmp 9 elisa kit, by R&D Systems (1 mentions)
Transwell chamber, by Merck Group (1 mentions)
Trypsin edta, by Merck Group (1 mentions)
6 protocols using western blot related chemical reagents
1
Cell Proliferation and Apoptosis Analysis
2
Chemoresistance Mechanisms in A549 Cells
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A549 and A549/DDP cell lines were purchased from the ATCC Cell Bank (USA). DMEM medium, fetal bovine serum (FBS), EDTA, and penicillin-streptomycin were bought from Hyclone (USA). Dimethyl sulfoxide and MTT powder were from Gibco. Tyresin-EDTA was from Sigma (USA). PVDF membrane was from Pall Life Sciences. Western blot-related chemical reagents were obtained from Beyotime. ECL reagent was from Amersham Biosciences. ERCC1, BRCA1, PI3K, AKT, phosphorylated PI3K, and phosphorylated AKT monoclonal antibodies, and HRP-tagged IgG secondary antibody were from Cell Signaling. Caspase 3 activity kits were purchased from Pall Life Sciences. RNA extraction kits and reverse transcription kits were from Axygen (USA). Other common reagents were purchased from Sangon. The Labsystem version 1.3.1 microplate reader was purchased from Bio-Rad (USA).
3
Comprehensive Cell Culture Protocol
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DMEM medium, fetal bovine serum (FBS), EDTA, and penicillin-streptomycin were purchased from Hyclone; dimethyl sulfoxide and MTT were purchased from Gibco; trypsin-EDTA was purchased from Sigma; PVDF membrane was purchased from Pall Life Sciences; Western blot-related chemical reagents were from Shanghai Beyotime biotechnology Co. LTD; ECL reagent was purchased from Amersham Biosciences; DDR-1 monoclonal antibody and HRP tagged IgG secondary antibody were purchased from Cell Signaling; nilotinib and transwell chambers were purchased from Sigma; MMP-2 and MMP-9 ELISA kits were purchased from R&D; other reagents were purchased from Shanghai Sangon Biotechnology Co., LTD; and Labsystem Version1.3.1 microplate reader was purchased from Bio-rad.
4
Protein Expression and Detection Workflow
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TRIzol reagent was from Invitrogen. Polyvinylidene difluoride (PVDF) membrane was from Pall Life Sciences. Western blot-related chemical reagents were bought from Beyotime. ECL reagent was from Amersham Biosciences. Mouse antihuman p53 primary antibody, mouse antihuman MDM2 primary antibody, and goat anti-mouse horseradish peroxidase (HRP)-tagged IgG secondary antibody were from Cell Signaling. The SABC immunohistochemical staining kit was purchased from Boster Bio-engineering, Ltd. (Wuhan, China). The RNA extraction kit and reverse transcription kit were from Invitrogen. DNA amplifier was from PE Gene Amp PCR System2400. Other common reagents were purchased from Sangon.
5
MCF-7 Cell Cytotoxicity Assay Protocol
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MCF-7 cell line was bought from the cell bank of Chinese academy of sciences. LA, paclitaxel, and enzyme-EDTA were obtained from Sigma Aldrich (St. Louis, MO, USA). DMEM medium was purchased from Gibco (Carlsbad, CA, USA). Fetal bovine serum (FBS) and penicillinstreptomycin were from Hyclone (Logan, Utah, USA). PVDF membrane was bought from Pall Life Sciences (Port Washington, NY, USA). MTT reagents, dimethyl sulfoxide, and western blot-related chemical reagents were from Shanghai Beyotime Biotechnology Co., Ltd. (Shanghai, China). ECL reagent was obtained from Amersham Biosciences (Piscataway, NJ, USA). NF-κB primary antibody and HRP tag IgG second antibody were bought from Cell Signaling (Danvers, Massachusetts, USA). Other reagents were purchased from Shanghai Sangon Biotechnology Co., Ltd. (Shanghai, China). Labsystem Version 1.3.1 microplate reader was bought from Bio-rad (Hercules, CA, USA).
6
Comprehensive Molecular Assay Protocol
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PVDF transfer membranes were bought from Pall Life Sciences (Port Washington, NY, USA). EDTA was purchased from GE Healthcare (Logan, UT, USA). Western blot-related chemical reagents were purchased from Shanghai Beyotime Biotechnology Co., Ltd. The ECL reagent was bought from GE Healthcare. The TRIzol reagent, RNA extraction kit, RT-PCR primers, reverse transcription (RT) kit, and real-time PCR reagents were bought from Invitrogen, USA. The CIRP primary antibody was purchased from DPC Biermann GmbH. The horseradish peroxidase (HRP)-conjugated IgG secondary antibody was bought from Cell Signaling Technology, USA. β-actin was purchased from Santa Cruz Biotechnology, USA. The ELISA kit was bought from R&D Systems, USA.
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