Cell culture supernatant was concentrated using Amicon Ultra 10 kDa (Sigma) and these protein samples were electrophoresed on 4–20% SDS-polyacrylamide gels and transferred to nitrocellulose membrane (Merck Millipore). The membranes were incubated with primary antibodies against mIFNβ (#97450) and TRAIL (#3219) (Cell Signaling Technology), followed by peroxidase-linked secondary antibody (#7074, Cell Signaling Technology). The membrane was probed with secondary antibodies and developed with ECL (Thermo Fisher Scientific). Equal loading of the supernatant samples was confirmed with the Ponceau S solution (Sigma).
Peroxidase linked secondary antibody
Manufactured by Cell Signaling Technology
Peroxidase-linked secondary antibody is a laboratory reagent used in Western blotting and other immunoassays. It functions as a detection system, binding to a primary antibody and generating a colorimetric or chemiluminescent signal when exposed to a suitable substrate.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using peroxidase linked secondary antibody
1
Immunoblotting of IFNβ and TRAIL
2
Immunoblotting of IFNβ and TRAIL
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Cell culture supernatant was concentrated using Amicon Ultra 10 kDa (Sigma) and these protein samples were electrophoresed on 4–20% SDS-polyacrylamide gels and transferred to nitrocellulose membrane (Merck Millipore). The membranes were incubated with primary antibodies against mIFNβ (#97450) and TRAIL (#3219) (Cell Signaling Technology), followed by peroxidase-linked secondary antibody (#7074, Cell Signaling Technology). The membrane was probed with secondary antibodies and developed with ECL (Thermo Fisher Scientific). Equal loading of the supernatant samples was confirmed with the Ponceau S solution (Sigma).
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