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Aav9 ef1a flex tva mcherry

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AAV9-EF1a-FLEX-TVA-mCherry is a viral vector product designed for the expression of the TVA receptor and mCherry fluorescent protein in target cells. The vector is based on adeno-associated virus serotype 9 (AAV9) and utilizes the EF1a promoter for expression. The FLEX (flip-excision) system allows for Cre-dependent expression of the transgenes.

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Lab products found in correlation

Aav9 cag flex rg, by Addgene (5 mentions) Enva sad δg egfp, by Addgene (5 mentions) Aav2 retro cag icre, by Addgene (3 mentions) Aav5 syn flex chrimsonr tdtomato, by Addgene (3 mentions) Aav5 hsyn flex chrimsonr tdtomato, by Addgene (2 mentions) Aav9 cag flex tdtomato, by Addgene (2 mentions) Aav9 syn flex jgcamp7s wpre, by Addgene (2 mentions) Aav2 ef1a flex off hchr2 h134r eyfp, by Vector Biolabs (2 mentions) Aav2 ef1a flex off hm3dq mcherry, by Vector Biolabs (2 mentions) Aav2 hsyn dio hm3d gq mcherry, by Addgene (1 mentions) Aav2 hsyn dio hm4di mcherry, by Addgene (1 mentions)

5 protocols using aav9 ef1a flex tva mcherry

1

Viral Vector Production and Characterization

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AAV9-hSyn-Flex-GCaMP6s-WPRE-SV40 was produced by the Vector Core of the University of Pennsylvania. AAV9-EF1α-DIO-eNpHR3.0-mCherry, AAV2-CaMKII2α-eNpHR3.0-mCherry, AAV2-CaMKII2α-mCherry, AAV2-EF1α-DIO-hChR2(H134R)-YFP-WPRE and AAV2-EF1α-DIO-mCherry were produced by the Vector Core of the University of North Carolina. AAV5-hSyn-FLEX-ChrimsonR-tdTomato (Addgene plasmid 62723), AAV9-CAG-FLEX-tdTomato (Addgene plasmid # 28306) and AAV9-Syn-Flex-jGCaMP7s-WPRE (Addgene plasmid # 104491) were purchased from Addgene. AAV2(retro)-CAG-iCre (Addgene plasmid # 81070) was produced by Vector Biolabs. AAV9-EF1a-FLEX-TVA-mCherry (Addgene plasmid # 38044) and AAV9-CAG-FLEX-RG (Addgene plasmid # 38043) were produced by Vigene Biosciences, Inc. EnvA-SAD-ΔG-eGFP (Addgene plasmid # 32635) was produced by the Viral Vector Core of the Salk Institute for Biological Studies. All viral vectors were stored in aliquots at −80°C until use.
Ma J., du Hoffmann J., Kindel M., Sofia Beas B., Chudasama Y, & Penzo M.A. (2021). Divergent projections of the paraventricular nucleus of the thalamus mediate the selection of passive and active defensive behaviors. Nature neuroscience, 24(10), 1429-1440.
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2

Viral Vector Production and Characterization

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AAV9-hSyn-Flex-GCaMP6s-WPRE-SV40 was produced by the Vector Core of the University of Pennsylvania. AAV9-EF1α-DIO-eNpHR3.0-mCherry, AAV2-CaMKII2α-eNpHR3.0-mCherry, AAV2-CaMKII2α-mCherry, AAV2-EF1α-DIO-hChR2(H134R)-YFP-WPRE and AAV2-EF1α-DIO-mCherry were produced by the Vector Core of the University of North Carolina. AAV5-hSyn-FLEX-ChrimsonR-tdTomato (Addgene plasmid 62723), AAV9-CAG-FLEX-tdTomato (Addgene plasmid # 28306) and AAV9-Syn-Flex-jGCaMP7s-WPRE (Addgene plasmid # 104491) were purchased from Addgene. AAV2(retro)-CAG-iCre (Addgene plasmid # 81070) was produced by Vector Biolabs. AAV9-EF1a-FLEX-TVA-mCherry (Addgene plasmid # 38044) and AAV9-CAG-FLEX-RG (Addgene plasmid # 38043) were produced by Vigene Biosciences, Inc. EnvA-SAD-ΔG-eGFP (Addgene plasmid # 32635) was produced by the Viral Vector Core of the Salk Institute for Biological Studies. All viral vectors were stored in aliquots at −80°C until use.
Ma J., du Hoffmann J., Kindel M., Sofia Beas B., Chudasama Y, & Penzo M.A. (2021). Divergent projections of the paraventricular nucleus of the thalamus mediate the selection of passive and active defensive behaviors. Nature neuroscience, 24(10), 1429-1440.
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3

Viral Vector Delivery Techniques

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AAV9-Syn-Flex-GCaMP6s-WPRE-SV40 was produced by the Vector Core of the University of Pennsylvania. AAV5-Syn-FLEX-ChrimsonR-tdTomato (Addgene plasmid # 62723) was produced by Addgene. AAV2-EF1a-FLEX/OFF-hChR2(H134R)-eYFP and AAV2-EF1a-FLEX/OFF-hM3Dq-mCherry were produced by Vector Biolabs. AAV9-Syn-DO-GCaMP6s and AAV9-Syn-DO-oScarlett were produced by Charu Ramakrishnan (Deisseroth’s lab, Stanford University). CAV2-Cre was produced by the Adenovirus Vector Core of the Institut de Genetique Moleculaire de Montpellier. AAV9-EF1a-DIO-eNpHR3.0-mCherry, AAV2-CaMKIIa-mCherry, AAV2-EF1a-DIO-hChR2(H134R)-EYFP, and AAV2-EF1a-DIO-mCherry were produced by the Vector Core of the University of North Carolina. AAV9-EF1a-FLEX-TVA-mCherry (Addgene plasmid # 38044) and AAV9-CAG-FLEX-RG (Addgene plasmid # 38043) were produced by Vigene Biosciences, Inc. EnvA-SAD-ΔG-eGFP (Addgene plasmid # 32635) was produced by the Viral Vector Core of the Salk Institute for Biological Studies. All viral vectors were stored in aliquots at −80°C until use. The CreOFF viral vectors (DO and FLEX/OFF) used in this study are fully available from the authors upon request.
Gao C., Leng Y., Ma J., Rooke V., Rodriguez-Gonzalez S., Ramakrishnan C., Deisseroth K, & Penzo M.A. (2020). Two genetically, anatomically and functionally-distinct cell types segregate across the antero-posterior axis of the paraventricular thalamus. Nature neuroscience, 23(2), 217-228.
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4

Viral Vector Delivery Techniques

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AAV9-Syn-Flex-GCaMP6s-WPRE-SV40 was produced by the Vector Core of the University of Pennsylvania. AAV5-Syn-FLEX-ChrimsonR-tdTomato (Addgene plasmid # 62723) was produced by Addgene. AAV2-EF1a-FLEX/OFF-hChR2(H134R)-eYFP and AAV2-EF1a-FLEX/OFF-hM3Dq-mCherry were produced by Vector Biolabs. AAV9-Syn-DO-GCaMP6s and AAV9-Syn-DO-oScarlett were produced by Charu Ramakrishnan (Deisseroth’s lab, Stanford University). CAV2-Cre was produced by the Adenovirus Vector Core of the Institut de Genetique Moleculaire de Montpellier. AAV9-EF1a-DIO-eNpHR3.0-mCherry, AAV2-CaMKIIa-mCherry, AAV2-EF1a-DIO-hChR2(H134R)-EYFP, and AAV2-EF1a-DIO-mCherry were produced by the Vector Core of the University of North Carolina. AAV9-EF1a-FLEX-TVA-mCherry (Addgene plasmid # 38044) and AAV9-CAG-FLEX-RG (Addgene plasmid # 38043) were produced by Vigene Biosciences, Inc. EnvA-SAD-ΔG-eGFP (Addgene plasmid # 32635) was produced by the Viral Vector Core of the Salk Institute for Biological Studies. All viral vectors were stored in aliquots at −80°C until use. The CreOFF viral vectors (DO and FLEX/OFF) used in this study are fully available from the authors upon request.
Gao C., Leng Y., Ma J., Rooke V., Rodriguez-Gonzalez S., Ramakrishnan C., Deisseroth K, & Penzo M.A. (2020). Two genetically, anatomically and functionally-distinct cell types segregate across the antero-posterior axis of the paraventricular thalamus. Nature neuroscience, 23(2), 217-228.
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5

Viral Vector Toolkit for Neuroscience

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AAV2/5-Ef1a-DIO hChR2(E123T/T159C)-EYFP and AAV9-hSyn-FLEX-GCaMP6s-WPRE-SV40 were produced by the Vector Core of the University of Pennsylvania. AAV9-EF1a-DIO-eNpHR3.0-mCherry, AA9-CAG-FLEX-tdTomato, AV9-hSyn-DIO-mCherry-2A-SyneGFP and AAV2-Syn-DIO-GFP were produced by the Vector Core of the University of North Carolina. AAV5-Syn-FLEX-ChrimsonR-tdTomato (Addgene plasmid # 62723) and AAV2-hSyn-DIO-hM4Di-mCherry, AAV2-hSyn-DIO-hM3D(Gq)-mCherry were produced by Addgene. AAV2(retro)-CAG-iCre (Addgene plasmid # 81070) was produced by Vector Biolabs. AAV9-EF1a-FLEX-TVA-mCherry (Addgene plasmid # 38044) and AAV9-CAG-FLEX-RG (Addgene plasmid # 38043) were produced by Vigene Biosciences, Inc. EnvA-SAD-ΔG-eGFP (Addgene plasmid # 32635) was produced by the Viral Vector Core of the Salk Institute for Biological Studies. AAV2-retro-CAG-tdTomato-WPRE is a gift from Dr. Yuanyuan Liu at NIDCR. All viral vectors were stored in aliquots at −80°C until use.
Sofia Beas B., Gu X., Leng Y., Koita O., Rodriguez-Gonzalez S., Kindel M., Matikainen-Ankney B.A., Larsen R.S., Kravitz A.V., Hoon M.A, & Penzo M.A. (2020). A ventrolateral medulla-midline thalamic circuit for hypoglycemic feeding. Nature Communications, 11, 6218.
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