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Ammonia solution

Sourced in China

Ammonia solution is an aqueous solution of ammonia. It is a clear, colorless liquid with a pungent odor. The solution contains varying concentrations of ammonia, typically ranging from 5% to 30%. Ammonia solution is commonly used in various industrial and laboratory applications.

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5 protocols using ammonia solution

1

Cuttlebone-Derived Silica Nanoparticles

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Cuttlebone was provided by BoZhou Sea Medicines Procurement Co., Ltd., Bozhou, China. Dichloromethane, acetone, n-hexane, anhydrous ethanol, ammonia solution, tetraethyl silicate, heptadecafluoro-1,1,2,2,-tetrahydrodecyl dimethylchlorosilane, and dopamine hydrochloride were purchased from Macklin Biochemical Co., Ltd., Shanghai, China. Diesel oil was purchased from Sinopec gas station. Edible blend oil was purchased from Shandong Luhua Group Co., Ltd., Yantai, China.
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2

Synthesis of Graphene-Reinforced Nanocomposites

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Ethylene glycol (EG, ≥99%), ethanol (AR, ≥99.5%), iron(iii) chloride (FeCl3·6H2O, AR, 99%), and nickel chloride hexahydrate (NiCl2·6H2O, AR, 98%) were purchased from Aladdin Chemicals, China. Trisodium citrate dihydrate (AR, 99%), anhydrous sodium acetate (AR, ≥99%), melamine (99%), and polyethylene glycol (PEG, average Mn 4000) were purchased from Sigma-Aldrich Chemicals. Ammonia solution (NH3·H2O, 28 wt% in H2O) and tetraethyl orthosilicate (TEOS, reagent grade, 98%) were acquired from Shanghai Macklin Biochemical. Deionized (DI) water, which was used in various processes, was synthesized in our laboratory using a Shenzhen Pure Water No. 1 Water Treatment Technology system. The resistivity of the DI water used in this study was 18.25 mΩ cm−1. The graphene used in the samples was provided by Shanghai Aladdin Biochemical Technology.
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3

Synthesis of Transition Metal Complexes

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2-Methylimidazole (AR, ≥98%), Sodium diethyldithiocarbamate (AR, ≥98%), Cobalt nitrate hexahydrate (Co(NO3)2·6H2O, AR, ≥99.7%), Copper nitrate trihydrate (Cu(NO3)2·3H2O, AR, ≥99.7%), Sodium 3-diethyldithiocarbamate trihydrate (DDTC), Iron chloride hexahydrate (FeCl3·6H2O, 99%), sulfate heptahydrate (FeSO4·7H2O, ≥98.0%), ammonia solution (AR, 25.0%–28.0%) were obtained from Shanghai Macklin Biochemical Co., Ltd. Absolute methanol (AR, ≥99.5%), Absolute ethanol (AR, ≥99.5%) were purchased from Tianjin Zhiyuan Chemical Reagent Co., Ltd.
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4

Comprehensive Histological Staining Protocol

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Phosphate buffered saline (PBS), 0.5% Oil Red O, Nile red, 4’,6-diamidino-2-phenylindole (DAPI) at 10 μg/mL, Triton X-100, and the Hematoxylin and Eosin (H&E) staining kit were acquired from Solarbio Technology Ltd. (Beijing, China). Propylene glycol, glycerol, ammonia solution, acetone, tricaine, and the reverting blue solution were sourced from Macklin (Shanghai, China). Paraformaldehyde (PFA) was procured from J&K Scientific Ltd. (Shanghai, China). Xylene, hydrochloric acid ethanol differentiation solution, and neutral resin were obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). All reagents employed were of analytical grade.
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5

Anionic Pectin-Polyethyleneimine Hydrogel for Cell Culture

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High acyl anionic pectin (AP) was purchased from Henan Zhongtai (Zhengzhou, China). Cationic polyethyleneimine (CP, MW ​= ​70,000, 50 ​wt% in water), anhydrous ethanol, sodium hydroxide (NaOH), and ammonia solution (28 ​wt% in water) were obtained from Macklin (Shanghai, China). Copper chloride and dopamine hydrochloride were purchased from Aladdin (Shanghai, China). Agar powder, Luria-Bertani broth, ascorbic acid, and tryptone soya broth were purchased from Solarbio (Beijing, China). Fetal bovine serum (FBS), penicillin/streptomycin (P/S), trypsin-EDTA (0.25%), Dulbecco's modified Eagle's medium (DMEM), and phosphate-buffered saline (PBS, pH ​= ​7.4) were bought from Gibco (Grand Island, USA). L7012 Live/Dead BacLight Bacterial Viability Kit comprising SYTO9 and propidium iodide (PI) was purchased from Thermo Fisher Scientific (Waltham, USA). Cell Counting Kit-8 (CCK-8) was commercially available from Glpbio (Montclair, USA). Live/Dead Cell Viability Kit composed of Calcein-AM and PI was purchased from Yeasen (Shanghai, China). All chemical reagents were used as supplied without further purification.
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