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2 protocols using magnesium chloride mgcl2

1

Analytical Characterization of Tea Compounds

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(-)-Epicatechin gallate (ECG, chromatographically pure) and (-)-epigallocatechin gallate (EGCG, chromatographically pure) were purchased from Taiyo Greenpower Co., Ltd. (Wuxi, China). Mucin (from the porcine stomach), (-)-epigallocatechin (EGC), (-)-epicatechin (EC), caffeic acid, chlorogenic acid, gallic acid and rutin were purchased from Sigma-Aldrich (Shanghai, China). Potassium chloride (KCl), calcium chloride (CaCl2), magnesium chloride (MgCl2) and aluminum chloride (AlCl3) were all analytically pure and were obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). We used a Hach TL2300 Turbidimeter (Shanghai, China). Tea samples were obtained from the Tea Research Institute, Chinese Academy of Agricultural Sciences (Hangzhou, China). The brewing water used was pure water obtained from Hangzhou Wahaha Group Co., Ltd. (Hangzhou, China).
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2

Rapid Immunoassay for Tetracycline Detection

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Trimethoxyoctadecylsilane (C21H46O3Si), bovine serum albumin (BSA) and tetracycline hydrochloride (C22H24N2O8·HCl) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Ampicillin, penicillin, streptomycin, kanamycin, dipotassium phosphate (K2HPO4), monopotassium phosphate (KH2PO4), sodium chloride (NaCl) and magnesium chloride (MgCl2) were purchased from Sinopharm (Hong Kong, China). Tween-20 was purchased from Solarbio (Beijing, China). The filter paper was purchased from Whatman (Maidstone, UK). Tetracycline-BSA antigen and anti-tetracycline monoclonal antibody were purchased from Suzhou Kuaijiekang Co., Ltd. (Suzhou, China). Commercial tetracycline ELISA kit was purchased from Shenzhen Finder Biotech Co., Ltd. (Shenzhen, China). The heating plate (YH-946B) was purchased from Shanghai Yuxing Electronics Co., Ltd. (Shanghai, China). The inkjet printer (IP2780) was purchased from Canon (Tokyo, Japan).
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