Ir prestige 21

Ag-NPs were first analysed in a UV-Visible Spectrophotometer (Perkin Elmer, USA; Lambda-25) at the wavelength of 200–800nm which was equipped with “UV-Winlab” software that records and analyses the data [44 (link)]. The Ag+ ion reduction was surveilled by estimating the UV-Vis spectrum of reaction medium (sample) after blending the sample in trivial aliquant into 20 times distilled water to minimize noise present in a sample [7 ].
Further, the characterization of Ag-NPs was performed by various technologies such as dynamic light scattering (DLS) and particle size together with zeta potential analysis. The sample was diluted 4 times in distilled water and then analysed using DLS (Malvern Instruments, UK, Nano ZS) and zeta potential of Zetasizer Ver. 7.12 [44 (link)]. FESEM (Zeiss, Sigma 300) for Ag-NPs was also noted. FTIR (Shimadzu Corp., Japan, IR-Prestige 21) analysis was done by recording the spectra in a wavelength between 4000 and 400 cm⁻¹ using a model IR-Prestige 21. For characterization in XRD (Rigaku, Japan, SmartLab 9kW), 10–15 ml of sample was dried overnight in an oven at 55–60 °C.

The chemical composition and conformation of the TGs were analyzed by FTIR spectroscopy (IRPrestige-21, Shimadzu, Kyoto, Japan) under an attenuated total reflectance (ATR) model (IRPrestige-21, Shimadzu, Kyoto, Japan). All spectra were obtained between 4600 cm⁻¹ and 800 cm⁻¹, at a 4 cm⁻¹ resolution and an average of 50 scans. Three samples were used for CTGs and PTGs, respectively.

Novel hydrazones have been synthesized from natural lauric acid in a stepwise manner. All the reagents used were analytical grade and solvents were used without further purification. The reaction progress was monitored by TLC cards covered with silica gel (0.25 mm thickness). The NMR spectra were recorded using a Bruker-Avance 400 MHz spectrometer, while FTIR analysis was performed using the Shimadzu IR Prestige-21.