Auy220

Manufactured by Shimadzu

Sourced in Japan

The AUY220 is a UV-Vis spectrophotometer manufactured by Shimadzu. It is a compact and versatile instrument designed for a wide range of analytical applications in the laboratory. The AUY220 measures the absorbance or transmittance of light through a sample, providing quantitative data about the concentration or composition of the analyte.

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Lab products found in correlation

Sodium hydroxide (naoh), by Vetec (1 mentions) Na2haso4 7h2o, by Merck Group (1 mentions) Naaso2, by Merck Group (1 mentions) Hydrochloric acid (hcl), by Vetec (1 mentions) Himac cr22n, by Hitachi (1 mentions) Sorvall lynx 6000, by Thermo Fisher Scientific (1 mentions) U 2900, by Hitachi (1 mentions) Jsm 6390lv, by JEOL (1 mentions)

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AUY220 analytical balance (2 citations)

24 protocols using auy220

Paperboard Grammage Determination

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The uncoated (control) and coated paperboard grammage were determined according to the ASTM D646-96 methodology [32 ]. The samples (12.5 × 12.5 cm) were weighted using an analytical balance (AUY220, Shimadzu, Kyoto, Japan), and the grammage was calculated according to Equation (2).

G = \frac{M}{A}

where G = grammage (g/m²); M = mass of the paper (g); A = paperboard area (m²).

Naitzel T.D., Garcia V.A., Lourenço C.A., Vanin F.M., Yoshida C.M, & de Carvalho R.A. (2023). Properties of Paperboard Coated with Natural Polymers and Polymer Blends: Effect of the Number of Coating Layers. Foods, 12(14), 2745.

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Arsenic Speciation Analysis Protocol

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All reagents used in the experiments were of analytical grade and used without further purification. The reagents used were weighed on a Shimadzu analytical scale, model AUY220, to prepare the solutions. The solutions were prepared using ultrapure water (resistivity 18.0 MΩ cm) obtained by the Thermo Scientific Barnstead™ Nanopure™ system (Waltham, MA, USA).
The reagents Na₂HAsO₄·7H₂O (CAS: 10048-95-0) and NaAsO₂ (CAS: 7784-46-5) were provided by Sigma-Aldrich (St. Louis, MO, USA), and the reagents NaOH (CAS: 1310-73-2), HNO₃ (CAS: 7697-37-2), CH₃COOH (CAS: 64-19-7), and HCl (CAS: 7647-01-0) were purchased from Vetec (Brazil). Rhodium (Ra) (CAS: 7440-14-4) and multi-elementary solution were obtained from PerkinElmer (Waltham, MA, USA).

Faria M.C., Hott R.D., dos Santos M.J., Santos M.S., Andrade T.G., Bomfeti C.A., Rocha B.A., Barbosa F J.r, & Rodrigues J.L. (2023). Arsenic in Mining Areas: Environmental Contamination Routes. International Journal of Environmental Research and Public Health, 20(5), 4291.

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Preparation of Deep Eutectic Solvents

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The DES constituents were weighed in analytical balance (Shimadzu, AUY220, SP, Brazil) to produce choline chloride and propylene glycol at 1:2 M ratio (ChCl:Pro), and chlorine chloride and malic acid (ChCl:Ma) at 1:1 M ratio. The hydrogen bonds between choline chloride and the hydroxyl groups of HBD must be adequate to form the eutectic mixtures, which are dependent of the molar ratios, selected according to Dai et al. (2013) (link). Then, the mixtures were heated at 80 °C, at continuous stirring, by a type Dubnoff bath (Ethik technology, 304 TPA model, SP, Brazil), until homogeneous and transparent liquid solvents were observed. To decrease the ChCl:Pro and ChCl:Ma viscosities, enabling the handling by PLE unit and improving dissolution rates, the solvents were diluted in distilled water at concentrations from 5 to 55%.

Benvenutti L., Zielinski A.A, & Ferreira S.R. (2022). Pressurized aqueous solutions of deep eutectic solvent (DES): A green emergent extraction of anthocyanins from a Brazilian berry processing by-product. Food Chemistry: X, 13, 100236.

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Quantitative Biomolecule Analysis

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An ultra-performance liquid chromatography (Acquity, Waters Corporation, Milford, MA) system coupled with tandem mass spectrometry (AB Sciex Corp., USA), an analytical weighing balance (AUY 220, Shimadzu, Kyoto, Japan), a homogenizer (IKA-T18) and a high speed freezing centrifuge (Himac CR 22N, Hitachi) were the main instruments in the experiment.

Zhao F., Liu J., Xie D., Lv D, & Luo J. (2018). A novel and actual mode for study of soil degradation and transportation of difenoconazole in a mango field. RSC Advances, 8(16), 8671-8677.

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Phase Diagram Construction for Microemulsions

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A microemulsion is only obtained if the amounts of the components are carefully quantified in a mixture [18 ]. Thus, the preparation of a phase diagram is essential for choosing the concentration of each substance. For this study, bidistilled water, soy oil, and the surfactant Tween^® 80 were used. Nine points were made using the mass titration method with these three components, where the masses of the surfactant + oil binary were fixed, and water is titrated to it until there is a visual change in the system from clear to turbid or vice versa. The components were weighed using an analytical balance (AUY220—Shimadzu) and then placed in the magnetic stirrer with heating (SL—91, SOLAB model) at a temperature of 60 °C.

de Brito A.M., da Silva Camboim W., Rossi C.G., de Souza I.A, & Silva K.K. (2023). The Microemulsion with Solubilization of the Ethanolic Extract of the Leaves of Melão-de-São-Caetano (Momordica charantia) and Antibacterial Action. Journal of Functional Biomaterials, 14(7), 359.

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Lifespan and Fecundity Assay in Flies

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Flies of the same age were collected and reared at a low density of 20 flies (10 females and 10 males) per vial and 10 vials for each group at 25 °C. For life span assay, we counted the live flies every day during the experiment and transferred them to new vials every third day for body weight measurement, and 10 adult flies were together weighed (10 females or 10 males per sample) using an ultrasensitive scale (AUY220, SHIMADZU, Kyoto, Japan). For egg-laying assay, single females were transferred to a fresh tube and eggs were counted every day. For these assays, 10 replicates were performed in each group.

Wei J.J., Li X.J., Liu W., Chai X.J., Zhu X.Y., Sun P.H., Liu F., Zhao Y.K., Huang J.L., Liu Y.F, & Zhao S.T. (2023). Eucommia Polysaccharides Ameliorate Aging-Associated Gut Dysbiosis: A Potential Mechanism for Life Extension in Drosophila. International Journal of Molecular Sciences, 24(6), 5881.

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Fruit Yield and Quality Analysis

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The number of fruits per plant was obtained from fruit counts, and the frequency of green and mature fruits was also determined separately. Fruit average weight was determined after individual weighing of each fruit, using a semianalytical balance with a sensitivity of 0.01 g (AUY220, Shimadzu, Kyoto, Japan). Yield per plant corresponds to the total weight of fruits per plant. The determination of the soluble solids content (°Brix, which is the percentage of soluble solids by weight) in the fruits was measured with a digital temperature-compensated refractometer, model RTD 45 (Instrutherm®, São Paulo, Brazil). Six ripe fruits per plant were evaluated in five replicates per genotype.

Barbosa M.A.M., Chitwood D.H., Azevedo A.A., Araújo W.L., Ribeiro D.M., Peres L.E.P., Martins S.C.V, & Zsögön A. (2019). Bundle sheath extensions affect leaf structural and physiological plasticity in response to irradiance. Plant, cell & environment, 42(5).

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Analytical Instrumentation for Spectrophotometry

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The instruments used in this study were as follows: UV1800 UV-Vis Spectrophotometer Shanghai Precision Scientific Instrument Co., Ltd., AuY220 Electronic Analytical Balance Japan Shimadzu Corporation; ZHWY-2102C Constant Temperature Cultivation Shaker Shanghai Zhi Cheng Analytical Scientific Instrument Co., Ltd., Sorvall LYNX6000 refrigerated centrifuge, American Thermo fisher company.

Luo X., Tian M., Cheng Y., Ji C., Hu S., Liu H., Lu J, & Ren J. (2022). Effects of simulated in vitro gastrointestinal digestion on antioxidant activities and potential bioaccessibility of phenolic compounds from K. coccinea fruits. Frontiers in Nutrition, 9, 1024651.

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NMN Quantification in Plant Extracts

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For the preparation of standards, NMN standards were weighed using an analytical balance (AUY220, SHIMADZU, Kyoto, Japan). The NMN standard used for PEL screening was dissolved in 10% DMSO solvent and serially diluted to 30, 25, 20, 15, 10, 5, 1, and 0.1 ng/mL control solution. For the measurement of C. verum, C. loureiroi, and Cabbage plant extract samples, NMN standard was dissolved in 25% ethanol, serially diluted to 100, 80, 40, 20, 10, 1, and 0.5 ng/mL control solutions. These standards were used to generate calibration curves to measure NMN concentrations in PEL and plant extracts.

Yan J., Sakamoto T., Islam A., Ping Y., Oyama S., Fuchino H., Kawakami H., Yoshimatsu K., Kahyo T, & Setou M. (2022). Cinnamomum verum J. Presl Bark Contains High Contents of Nicotinamide Mononucleotide. Molecules, 27(20), 7054.

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Fruit Biometrics of Tomato Genotypes

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The productivity parameters were measured in eight plants per genotype (WT, ARF4-as and SlARF4-crispr). The average fruit weight was determined after individual weighing of each fruit, using a semi analytical balance with a sensitivity of 0.01 g (Shimadzu^® AUY220 model, Japan)). Equatorial and polar diameter was measured using a digital pachymeter (Jomarca STAINLESS HARDENED, Nieuw, Vennep, Netherland). The determination of the soluble solids content (°Brix) was performed in 10 fruits per plant using a digital temperature-compensated refractometer, model RTD 45 (Instrutherm^®, São Paulo, SP, USA).

Bouzroud S., Gasparini K., Hu G., Barbosa M.A., Rosa B.L., Fahr M., Bendaou N., Bouzayen M., Zsögön A., Smouni A, & Zouine M. (2020). Down Regulation and Loss of Auxin Response Factor 4 Function Using CRISPR/Cas9 Alters Plant Growth, Stomatal Function and Improves Tomato Tolerance to Salinity and Osmotic Stress. Genes, 11(3), 272.

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