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Flpo mice

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FLPo Mice are a specialized strain of laboratory mice developed by Jackson ImmunoResearch. They carry a gene encoding the FLPo recombinase, a highly efficient site-specific DNA recombinase. This recombinase can be used to induce genetic modifications in target cells or tissues, enabling researchers to study various biological processes.

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Lab products found in correlation

Rosa26sorcag tdtomato ai14, by Jackson ImmunoResearch (2 mentions) Foxp3yfp cre, by Jackson ImmunoResearch (2 mentions) Foxp3egfp creert2, by Jackson ImmunoResearch (2 mentions) Myf5 cre mice, by Jackson ImmunoResearch (1 mentions)

Spelling variants of this product

PGK1-FLPo mice (2 citations) FLPo deleter mice (2 citations)

3 protocols using flpo mice

1

Conditional Knockout of Mitochondrial Proteins

Cited 1 time
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C57BL/6 Uqcrfsfl/fl (RISPfl/fl) are previously described8 (link). C57BL/6 mice harboring a loxp-flanked exon 1 of the Uqcrq gene (encodes QPC, QPCfl/fl mice) were generated by Ozgene (locus map in Extended Data Figure 4a). The frt-flanked neomycin resistance cassette was removed by crossing QPCfl/fl mice to mice expressing FLP-recombinase (FLPo Mice Jackson Lab, Stock no. 011065). Loss of the Neo-cassette was confirmed using PCR. QPCfl/fl mice were genotyped using the following primers: 1. 5’-CTTCCGCTCCTCCCGGAAGT-3’, 2. 5’-TTCCCAAACTCGCGGCCCATG-3’ and 3. 5’-CAATTCCAGCCAACAGTCCC-3’ which allow identification of the Uqcrq wild-type, loxp-flanked, and excised alleles. RISPfl/fl and QPCfl/fl mice were checked for C57BL/6 status and both are >99% C57BL/J using the genome scanning service from Jackson Laboratories. Foxp3YFP-Cre (stock no. 016959), Foxp3eGFP-Cr-eERT2 (stock no. 016961), and ROSA26SorCAG-tdTomato (Ai14, stock no. 007908) mice were obtained from Jackson Laboratory. 40 mg/ml tamoxifen dissolved in corn oil was administered to mice every third day using oral gavage to reach an effective dose of 320mg/kg in 8 to 12-week old mice. All animal procedures were approved by Institutional Animal Care and Use Committee (IACUC) at Northwestern University.
Weinberg S.E., Singer B.D., Steinert E.M., Martinez C.A., Mehta M.M., Martínez-Reyes I., Gao P., Helmin K.A., Abdala-Valencia H., Sena L.A., Schumacker P.T., Turka L.A, & Chandel N.S. (2019). Mitochondrial complex III is essential for regulatory T cell suppressive function. Nature, 565(7740), 495-499.
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2

Generation and Analysis of Grsf1 Knockout Mice

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The generation and analysis of the mutant mice were approved by the Animal Care and Use Committee of the National Institute on Aging. C57BL/6N-Atm1Brd Grsf1tm1a(EUCOMM)Wtsi/WtsiIeg (Grsf1tm1a) mice harboring a mutant allele in the Grsf1 locus were purchased from the European Mouse Mutant Archive (EMMA, EM:10950). In the mutant allele, exons 4 and 5 of Grsf1 were floxed by two LoxP sites (Figure 1A). Additionally, a LacZ and a Neo cassette, sandwiched by two FRT (Flippase recognition target) sites were inserted into intron 3. To generate conditional Grsf1-LoxP mice, we deleted the LacZ and Neo cassettes by crossing Grsf1tm1a mice with FLPo mice (Stock No: 007844, Jackson Laboratory) expressing the Flippase widely under the Gt(ROSA)26Sor promoter. The resultant Grsf1-LoxP (Grsf1 floxed) mice were crossed further with the Myf5Cre mice (Stock No: 007893, the Jackson Laboratory) to generate skeletal muscle-specific Grsf1 knockout (Grsf1cKO) mice (Figure 1A). Genotyping was done by Transnetyx (Cordova, TN) following protocols from EMMA and Jackson Laboratories.
Driscoll R.K., Krasniewski L.K., Cockey S.G., Yang J.H., Piao Y., Lehrmann E., Zhang Y., Michel M., Noh J.H., Cui C.Y, & Gorospe M. (2021). GRSF1 deficiency in skeletal muscle reduces endurance in aged mice. Aging (Albany NY), 13(11), 14557-14570.
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3

Conditional Knockout of Mitochondrial Proteins

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
C57BL/6 Uqcrfsfl/fl (RISPfl/fl) are previously described8 (link). C57BL/6 mice harboring a loxp-flanked exon 1 of the Uqcrq gene (encodes QPC, QPCfl/fl mice) were generated by Ozgene (locus map in Extended Data Figure 4a). The frt-flanked neomycin resistance cassette was removed by crossing QPCfl/fl mice to mice expressing FLP-recombinase (FLPo Mice Jackson Lab, Stock no. 011065). Loss of the Neo-cassette was confirmed using PCR. QPCfl/fl mice were genotyped using the following primers: 1. 5’-CTTCCGCTCCTCCCGGAAGT-3’, 2. 5’-TTCCCAAACTCGCGGCCCATG-3’ and 3. 5’-CAATTCCAGCCAACAGTCCC-3’ which allow identification of the Uqcrq wild-type, loxp-flanked, and excised alleles. RISPfl/fl and QPCfl/fl mice were checked for C57BL/6 status and both are >99% C57BL/J using the genome scanning service from Jackson Laboratories. Foxp3YFP-Cre (stock no. 016959), Foxp3eGFP-Cr-eERT2 (stock no. 016961), and ROSA26SorCAG-tdTomato (Ai14, stock no. 007908) mice were obtained from Jackson Laboratory. 40 mg/ml tamoxifen dissolved in corn oil was administered to mice every third day using oral gavage to reach an effective dose of 320mg/kg in 8 to 12-week old mice. All animal procedures were approved by Institutional Animal Care and Use Committee (IACUC) at Northwestern University.
Weinberg S.E., Singer B.D., Steinert E.M., Martinez C.A., Mehta M.M., Martínez-Reyes I., Gao P., Helmin K.A., Abdala-Valencia H., Sena L.A., Schumacker P.T., Turka L.A, & Chandel N.S. (2019). Mitochondrial complex III is essential for regulatory T cell suppressive function. Nature, 565(7740), 495-499.
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