Cd31 apc clone wm59

Manufactured by BioLegend

Sourced in United States

CD31-APC clone WM59 is a fluorochrome-conjugated monoclonal antibody that recognizes the CD31 antigen. CD31, also known as PECAM-1, is a cell adhesion molecule expressed on the surface of various cell types, including endothelial cells, platelets, and certain leukocytes. This product can be used for the identification and enumeration of CD31-positive cells in flow cytometry applications.

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Lab products found in correlation

Cd73 pe clone rea804, by Miltenyi Biotec (2 mentions) Cd90 fitc clone rea897, by Miltenyi Biotec (2 mentions) Cd45 pe vio770 clone rea747, by Miltenyi Biotec (2 mentions) Cytoflex flow cytometer, by Beckman Coulter (2 mentions)

2 protocols using cd31 apc clone wm59

Flow cytometry analysis of ASCs

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ASCs at passage 1 were analyzed by flow cytometry to score the expression of MSC (CD73-PE clone REA804, CD90-FITC clone REA897; Miltenyi Biotec, Bergisch Gladbach, Germany) and hemato-endothelial (CD45-PE Vio770 clone REA747; Miltenyi Biotec. CD31-APC clone WM59; Biolegend, San Diego, CA, USA) markers. A minimum of 30,000 events were acquired with a CytoFLEX flow cytometer (Beckman Coulter, Fullerton, CA, USA).

Ragni E., Piccolo S., De Luca P., Taiana M., Grieco G, & de Girolamo L. (2024). Housekeeping Gene Stability in Adipose Mesenchymal Stromal Cells Cultivated in Serum/Xeno-Free Media for Osteoarthritis. Cells, 13(2), 167.

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Multiparametric Characterization of Stem Cells

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ASCs, BMSCs, and hAMSCs at passage 1 were analyzed by flow cytometry to score the expression of hemato-endothelial (CD45-PE Vio770 clone REA747; Miltenyi Biotec, Bergisch Gladbach, Germany. CD31-APC clone WM59; Biolegend, San Diego, CA, USA) and MSC (CD73-PE clone REA804 and CD90-FITC clone REA897; Miltenyi Biotec, Bergisch Gladbach, Germany) markers. A minimum of 30,000 events were acquired with a CytoFLEX flow cytometer (Beckman Coulter, Fullerton, CA, USA).

Ragni E., Piccolo S., Papait A., De Luca P., Taiana M., Grieco G., Silini A.R., Parolini O, & de Girolamo L. (2024). Stable Housekeeping Genes in Bone Marrow, Adipose Tissue, and Amniotic Membrane-Derived Mesenchymal Stromal Cells for Orthopedic Regenerative Medicine Approaches. International Journal of Molecular Sciences, 25(3), 1461.

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