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13 protocols using pentanol

1

Volatile Compound Analysis of Wheat and Buckwheat Flour

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Wheat and buckwheat flour was purchased from a local supermarket in Dalian, Liaoning, China. Standard chemicals: analytical grade (pentanal, hexanal, heptanal, octanal, nonanal, decanal, 1‐penten‐3‐ol, pentanol, hexanol, heptanol, octanol, toluene, ethyl benzene, p‐xylene, styrene, 2‐pentyl‐furan, and C4–C20 n‐alkanes) were purchased from Sigma‐Aldrich. Internal standards (tetracosane, p‐chloro‐L‐phenylalanine, 5R‐cholestan‐3β‐ol, Phenyl‐β‐D‐glucopyranoside) were purchased from Aladdin.
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2

Lipids and Fluorescent Probes for Membrane Studies

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Phosphocholine (PC) lipids including DOPC (dioleoyl-PC, or di-18:1-PC), DPPC (dipalmitoyl-PC or di-16:0-PC), POPC (palmitoyl-oleoyl-PC or 16:0-18:1-PC), di16:1-Δ9cis-PC, di18:1-Δ6cis-PC, di14:1-Δ9cis-PC, lyso(18:0)-PC, and palmitoyl sphingomyelin (PSM or 18:1-16:0 SM) were from Avanti Polar Lipids (Alabaster, AL). Texas Red dihexadecanoyl-phosphoethanolamine was from Life Technologies (Grand Island, NY), and cholesterol was from Sigma Aldrich (St. Louis, MO). Lipid structures appear in Fig. S1. Stock solutions of laurdan (Invitrogen, Carlsbad, CA) and C-laurdan (a gift from B. R. Cho, Seoul, Korea) were prepared in ethanol and dimethyl sulfoxide (DMSO). Alcohols (ethanol, propanol, butanol, pentanol, hexanol, octanol, decanol, tetradecanol, hexadecanol, propofol, and 2,6-di-tert-butylphenol), DMSO, and all additional reagents were from Sigma Aldrich unless specified. All alcohols were purchased at their highest available purity. All materials were used as from the manufacturer without further purification.
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3

Quantification of Fermentation Metabolites

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Methanol and acetonitrile (99% purity) were purchased from Aladdin Company (Shanghai, China). All standards used in this study, including lactic acid, propanoic acid, hexanoic acid, ethanol, Methanol, pentanol, n-propanol, ethyl lactate, ethyl acetate, and ethyl formate, were purchased from Sigma-Aldrich (St. Louis, MO, USA). All other chemicals and reagents used in this study were of analytical grade and were obtained from the Sinopharm Chemical Reagent Company (Beijing, China).
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4

Extraction and Characterization of Mimosa Tannin Powder

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The industrially extracted mimosa (Acacia mearnsii) tannin powder used in the current study was obtained from UCL Tannin Company Pty (Ltd), Kwa-Zulu Natal, South Africa. The extract was obtained from the bark of a Acacia mearnsii tree through a series of hot water extraction processes at a controlled temperature, pressure, and time, followed by vacuum evaporation and subsequent air-drying into fine powder before packaging and storage at −4 °C [16 (link)]. The molecular weight of the mimosa ranged from 500–3000 Daltons [24 (link)]. The organic solvents used were: ethyl acetate and pentanol, procured from Sigma Aldrich, Johannesburg, South Africa. According to Missio et al. [16 (link)], pentanol has a polarity index of 0.568, whereas ethyl acetate has a polarity index of 0.228. Tannic acid, Folin–Ciocalteu reagent, butanol, and all other reagents used were of analytical grades supplied from Sigma Aldrich, Johannesburg, South Africa.
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5

Volatile Compound Identification Protocol

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The standard compounds employed in this study for identification and quantification were supplied by Sigma-Aldrich (Darmstadt, Germany), including hexanal, (E)-2-hexenal, nonanal, (E,E)-2,4-hexadienal, (E)-2-nonenal, benzaldehyde, pentanol, hexanol, (Z)-3-hexen-1-ol, (E)-2-hexen-1-ol, 2-ethyl-1-hexanol, nonanol, benzyl alcohol, 2-phenylethanol, limonene, α-ocimene, linalool, citronellol, nerol, geraniol, and isovalerone. Sodium chloride and 4-methyl-2-pentanol (internal standard) were purchased from Merck (Darmstadt, Germany).
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6

Synthesis of EDOT-Based Polymer Nanostructures

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3,4-ethylenedioxythiophene (EDOT), iron (III) chloride, cetyltrimethylammonium bromide (CTAB) (≥98%), sodium chloride, toluene (>99%), pentanol (≥99%), silver perchlorate AgClO4 (>98%), phenol (C6H5OH) and methyl orange were purchased from Sigma-Aldrich. Titania (P25, surface area = 50 m2 g_1, 80% Anatase, 20% Rutile) was obtained from Evonik for comparative photo degradation by polymer nanostructure. N2 gas (purity > 99.995%) was purchased from Air Liquide. All compounds were used as received. Ultrapure water (Millipore System, 18.2 MΩ cm) and ethanol (≥99% for HPLC, purchased from Sigma-Aldrich) were used as solvents. All experiments were performed at room temperature.
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7

Zinc Microparticles Ink Formulation

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Zn microparticles (2 µm average diameter, Sigma Aldrich), polyvinylpyrrolidone (Sigma Aldrich, Mw = 360 K or 2000 K) and Pentanol (Sigma Aldrich) were mixed at varying weight ratios (25:1:5 for the final formulation). The ink was homogenized with a planetary mixer (Thinky ARE-250) at 300 rpm for 30 min. It was stored at 4 °C and brought to room temperature and homogenized at 300 rpm for 10 min before printing.
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8

Microbiological Analysis of Food and Beverages

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All chemicals used were of analytical grade. Potassium chromate, 0.1 N Sodium hydroxide, silver nitrate, methyl alcohol, ethyl alcohol, and sodium chloride were obtained from Duksan Pure Chemicals (Ansan, Gyeonggi-do, Korea). Sodium hydroxide, sodium hydrogen carbonate, and ammonium hydroxide were purchased from Junsei Chemicals (Tokyo, Japan). Formalin solution, standard methanol, ethanol, pentanol, propanol, and butanol were purchased from Sigma-Aldrich (St. Louis, MO, USA). Plate count agar, nutrient agar, potato dextrose agar (PDA), and potato dextrose broth were purchased from Difco (Becton, Dickinson and Company, Sparks, MD, USA). Mannitol egg yolk polymyxin agar (MYP), egg yolk emulsion, and polymyxin B supplement were purchased from Oxoid LTD (Basingstoke, Hampshire, UK). 3M Yeast and Mold Petrifilm was purchased from 3M Health Care (St. Paul, MN, USA). API 50CHB and API 20E were obtained from bioMerieux (Marcy I’Etoile, France).
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9

Soybean Oil Extraction and Characterization

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One kilogram of soybean seeds (HN‐48) was purchased from a local market in Dalian, Liaoning, China. The oil was extracted from the seeds using an expeller machine (HDC, Model LTP205). The extracted oils were filtered and stored in sealed glass bottles in the refrigerator (4°C) for further analysis. Standard chemicals: analytical grade (pentanal, hexanal, heptanal, octanal, nonanal, decanal, 1‐penten‐3‐ol, pentanol, hexanol, heptanol, octanol, toluene, ethyl benzene, p‐xylene, styrene, 2‐pentylfuran, and C4–C20 n‐alkanes) were purchased from Sigma‐Aldrich.
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10

Profiling Volatile Compounds in Milk

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Propionic acid, butanoic acid, pentanoic acid, hexanoic acid, heptanoic acid, octanoic acid, n-decanoic acid, hexanal, octanal, nonanal, (E)-2-octenal, pentanol, hexanol, 1-octen-3-ol, nonanol, paracresol, 2-heptanone, 3-octanone, 2-nononone, acetophenone, ethyl butyrate, ethyl caproate, and d-limonene were purchased from Sigma-Aldrich (St. Louis, MO). These chemical substances were detected to be the odor-active compounds of milk fan Y6 sample in our previous study (Tian et al., 2019) (link). All of the chemicals were of chromatographic grade.
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