Soybean lox 1

DHA and EPA were purchased from NuChek Prep, Inc (Elysian, MN). Soybean LOX-1 (lipoxidase, type V) was purchased from Sigma. C57BL/6NCrl mice (6–8 weeks) were purchased from Charles River Laboratories. Zymosan A was purchased from Sigma. We thank Professor Trond Hansen for providing a sample of synthetic PD1. PD1 and MaR1 were also purchased from Cayman Chemical.

The 12-aminododecanoic acid standard was purchased from Alfa Aesar (Haverhill, MA, USA), while the 12-oxo-9(Z)-dodecenoic acid and 12-oxo-10(E)-dodecenoic acid standards were purchased from Larodan (Solna, Sweden). Pyridoxal-5-phosphate monohydrate (PLP) was obtained from Acros Organics, Thermo Fisher Scientific (Waltham, MA, USA). Hexanal, hexylamine, soybean LOX-1, and L-lactate dehydrogenase (LDH) were supplied from Sigma Aldrich (St. Louis, MO, USA). Linoleic acid was obtained from Thermo Fisher Scientific (Waltham, MA, USA). β-Nicotine amide adenine dinucleotide disodium salt (NADH), isopropyl β-d-1-thiogalactopyranoside (IPTG), l-alanine, imidazole, ampicillin sodium salt, and kanamycin sulfate were purchased from Carl Roth (Karlsruhe, Germany). 13S-Hydroperoxy-9(Z),11(E)-octadecadienoic acid (13S-HPODE) was prepared from Linoleic acid by a peroxidation reaction with LOX-1 from Glycine max as described previously (Gala Marti et al. 2021 (link)). All other chemicals were from Sigma-Aldrich (St. Louis, MO, USA), Thermo Fisher Scientific (Waltham, MA, USA) or Carl Roth (Karlsruhe, Germany).