Transverse sections of 10 µm thick were obtained from paraffin-embedded aortic tissue and stained using the Sirius Red/Fast Green collagen staining kit to assess aortic fibrosis according to the manufacturer's guidelines (catalogue number 9046, Chondrex, Inc., Redmond, WA, USA). After staining, sections were mounted in DPX. Images were acquired using a Leica MC170 camera connected to a Leica DM2000 LED microscope (Leica, Diegem, Belgium). The area of collagen deposition, indicated by red staining, was outlined in at least four randomly chosen regions. The collagen-positive area was normalized to the total aortic area and expressed as % collagen content. Intima-media thickness was measured as the distance from endothelium to media-adventitia transition and expressed in µm. Both collagen deposition and intima-media thickness were quantified with ImageJ21 (link).
Mc170
Manufactured by Leica
Sourced in Germany, Belgium
The MC170 is a digital microscope camera produced by Leica. It is designed to capture high-quality images and videos of specimens under microscopic observation. The camera features a CMOS sensor and various image capture settings to suit different research and analysis needs.
Automatically generated - may contain errors
Lab products found in correlation
Dm750p, by Leica (2 mentions)
Dm2000 led microscope, by Leica (2 mentions)
Sirius red fast green collagen staining kit, by Chondrex (2 mentions)
S120vc, by Thorlabs (1 mentions)
Quanta 250 feg, by Thermo Fisher Scientific (1 mentions)
Z16 apo, by Leica (1 mentions)
H7752, by Merck Group (1 mentions)
M205c stereomicroscope, by Leica (1 mentions)
Dm 1000 led microscope, by Leica (1 mentions)
12 protocols using mc170
1
Quantifying Aortic Fibrosis and Intima-Media Thickness
2
Quantifying Prey-Predator Interactions in C. elegans and P. pacificus
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Caenorhabditis elegans larvae were collected as described in the Corpse Assay . One microliter of the prey pellet was seeded onto 35 mm NGM plates. Twenty to thirty P. pacificus hermaphrodites (1- to 2-day adult) were placed on the assay plates. After 15 min of recovery, the NGM agar containing worms was removed from the dishes and placed on a glass slide. Pharyngeal pumping and tooth movement were observed at 400× magnification and recorded using a charge-coupled device (CCD) camera (Leica, MC170) at 30 fps. We could not count the number of tooth movements in 15 s, as previously described (Okumura et al. 2017 (link)), because the biting events of Ppa-nas-6 mutants rarely lasted for 15 s. Instead, we counted the number of tooth movements per 20 pumping events.
3
Characterization of AKD Emulsion Morphology
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4
Mapping 2D Optical Retardation
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2D retardation maps were obtained using a polarized optical microscope (DM750P, Leica) with a multi-color CCD camera (MC170, Leica), which has a 1024×768 pixels resolution. An automatic precision stage (TI2-S-HIU, Nikon) was used to align each measurement to the correct position. The transmittance intensity was measured using a power meter (S120VC, Thorlabs). The maskless lithography setup used in our previous work was used to fabricate pixelated wrinkles corresponding to the pre-set keys in Fig. 5a .
5
Dytiscus marginalis Parasites in Turkey
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Parasitized specimens of Dytiscus marginalis 39°09'23"N, 41°34'56"E 1 ). The coordinates and altitude information of the locality were taken directly from a handheld GPS tool (Magellan Explorist 610). The beetles were collected with a net of mesh size 0.5 mm diameter. Specimens were fixed and preserved in 70% ethyl alcohol solution at the collection site. The clay and muddy substance on their surfaces was brushed off with a small paint brush in the laboratory and each specimen was checked for the presence of water mites under a stereomicroscope. Beetle species were identified according to Friday (1988) , Nilsson and Holmen (1995) , Pederzani (1995) , and Nilsson (1996) and mite larvae according to Wainstein (1980) . Photographs were taken using stereo microscope (Z16 APO; Leica, Wetzlar, Germany) equipped with an HD camera (Leica MC170), and with a scanning electron microscope (Quanta 250 FEG; FEI, Eindhoven, Netherlands). The examined material is deposited in the private collection of the first author, at Dicle University, Diyarbakır, Turkey.
The following abbreviations are used:Cx L W
The following abbreviations are used:
6
Oral Toxicity Assessment of NanoR
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The oral toxicity of NanoR was studied. ICR mice were randomly divided as follows: control group, vehicle group, low dose NanoR (25 mg/kg/day), and high dose NanoR (50 mg/kg/day) containing five animals in each group. At the end of the 15-day continuous treatment period, the organs of each mouse were collected and fixed in 4% paraformaldehyde, including heart, lung, liver, kidney, and spleen. The fixed organs were embedded in paraffin, sliced into sections with a thickness of 6 μm, and stained with hematoxylin–eosin (H&E). The stained slides were photographed using a light microscope (Leica MC170, Wetzlar, Germany).
7
Hermaphrodite Pharyngeal Pumping Analysis
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Ten to 20 adult hermaphrodites (1- to 2-day-old) were placed on E. coli lawns seeded on 35 mm NGM plates. NGM agar was removed from the dish and placed on a glass slide. Pharyngeal pumping was observed at 200× magnification and recorded using a CCD camera (Leica, MC170) at 30 fps for 15 s. Animals with both Eu and St forms were recorded and analyzed separately.
8
Serotonin-Induced Tooth Movement in Worms
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Worms (1- to 2-day-old) were collected from a 6 cm NGM plate and washed 3 times. Serotonin creatinine sulfate monohydrate (10 mg/mL; Sigma, H7752) was added to the worm pellet and incubated for 10 min at 20 to 25°C. Worms were loaded onto NGM plates, and a cover glass was placed. Tooth movement was observed at 400× magnification and recorded using a CCD camera (Leica, MC170) at 30 fps for 15 s.
9
Standardized Arachnid Specimen Analysis
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All specimens were preserved in 75% ethanol and examined, illustrated, photographed and measured using a Leica M205C stereo microscope equipped with a drawing tube, a Leica MC170 camera and LAS software (Ver. 4.8) . Male palps and female epigynes were examined and illustrated after being dissected. Female genitalia were cleared in 90% lactic acid. Eye sizes were measured as the maximum dorsal diameter. Leg measurements are shown as: total length (femur, patella and tibia, metatarsus, tarsus). All measurements are in millimeters. Specimens are deposited at the College of Life Sciences, Hunan Normal University, Changsha, China (HNU). Morphological terminology for descriptions and figures follows Álvarez-Padilla & Hormiga (2011) (link). 12E).
10
Galα1,3Gal Antigen Detection in Pig Tissues
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Tissue sections from different organs of pigs (six controls and six modified animals) were harvested immediately following termination of the animals, cut into smaller fragments and transferred to buffered 10% formalin. After several days of fixation in formalin, all tissue samples were embedded in paraffin using a standard procedure. For detection of Galα1,3Gal antigen, a primary monoclonal anti-Galα1,3Gal antibody (MyBiosurce) with streptavidin-biotinylated complex method using the Novolink Polymer Detection System was applied. The reaction was visualized with 3-3' diaminobenzidine (DAB). The studies were conducted on serial, 4 μm paraffin sections, on SuperFrost/ Plus microscopic slides. Fixed specimens were exam-ined using a Leica DM 1000 Led microscope with a Leica Mc170 digital camera.
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