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2 protocols using oltipraz

1

Ferroptosis Inducers and Inhibitors

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Penicillin, trypsin‐EDTA, streptomycin, and dimethyl sulfoxide (DMSO) were obtained from Sigma‐Aldrich (St. Louis, MO, USA). RIPA lysis buffer and bovine serum albumin (BSA) were purchased from Beyotime (Shanghai, China). Protease inhibitor cocktail, phosphatase inhibitor cocktail, 4’,6‐diamidino‐2‐phenylindole (DAPI) dye, and trypsin‐EDTA were acquired from Thermo Fisher Scientific (Scoresby, Vic, Australia). Minimum essential medium α (α‐MEM) and foetal bovine serum (FBS) were acquired from Gibco (Grand Island, NY, USA). Ferrostatin‐1 (MF: C15H22N2O2, MW: 262.35), RSL3 (MF: C23H21CIN2O5, MF: 440.88) and Oltipraz (MF: C8H6N2S3, MW: 226.34) were purchased from MedChem Express (Shanghai, China).
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2

Investigating Nrf2 Pathway Regulation

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Metformin was purchased from Sigma–Aldrich. Nrf2 pathway activator oltipraz and inhibitor ML385 were obtained from MedChemExpress. Antibody for HMGCS1 was purchased from Abcam (catalog no.: ab194971). The anti-NRF2 antibody was purchased from Proteintech (catalog no.: 16396-1-AP) and Abcam (catalog no.: ab62352). The anti-phosphor-NRF2 antibody was purchased from Abcam (catalog no.: ab76026). The antibodies against GAPDH (catalog no.: 3683; Cell Signaling Technology), tubulin (catalog no.: 5346; Cell Signaling Technology), and histone H3 (catalog no.: AH433; Beyotime) served as internal references for Western blots.
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